急性脑静脉闭塞动物模型的建立及磁共振评价.pdfVIP

重庆医科大学学报2008年第33卷第4期 (Journal of Chongqing Medical University 2008．Vo1．33 No．4) 一 481一 文章编号：0253—3626(2008)04—048 1-05 急性脑静脉闭塞动物模型的建立及磁共振评价 郭大静 ，王志刚2赵建农 ，陈维娟 ，谢微波 ，钟维佳 (1．重庆医科大学附属第二医院放射科；2．重庆医科大学超声影像学研究所，重庆 400010) 【摘 要】目的：拟建立一种稳定的急性脑静脉闭塞动物模型，用磁共振DWI、PWI、MRS、DTI对模型进行评价，观察脑实质损害 的变化规律。方法：家猫30只，随机分为2组(手术组24只，假手术组6只)，手术组采用开颅上矢状窦穿刺注射醋酸纤维素聚 合物(Ce11ulose acetate polymer，CAP)制备急性脑静脉闭塞模型，假手术组仅行开颅上矢状窦暴露，术后1…3 6 12、24、48h对各 组模型行MRI检查。结果：手术组22只猫造模成功。DWI发现14只猫脑实质出现25个异常信号，PWI发现17只猫脑实质内 31个异常灌注区。MRS检查示病变区Lac术后1h升高明显，以后逐渐降低，术后3h病变区NAA开始降低。DTI检查示术后 lh，ADCav值降低，3h后逐渐增高，FA值于术后各时间点均降低。病理学检查见病灶以血管源性水肿为主，12h后出现静脉性脑 梗塞、脑出血。假手术组脑实质未见异常改变。结论：上矢状窦穿刺注射CAP制备急性脑静脉闭塞模型方法可行，该模型适合于 病理生理及影像学研究。DWI结合PWI，MRS、DTI对急性脑静脉闭塞脑实质损害的动态变化、发病机理的判断具有重要价值。 【关键词】模型；脑静脉闭塞；磁共振成像 【中国图书分类法分类号】R816．1 【文献标识码】A 【收稿日期】2007-07—30 Establishment of acute cerebraI venous occlusion and evaluation of it with magnetic resonance imaging GUO Da--jing，et al (Department of Radiology，the Second Affiliated Hospital，Chongqing Medical University) 【Abstract】Objective：To establish a stable animal model of acute cerebral venous occlusion and to evaluate the model with DWI．PWI，MRS and DTI in observing the changing regularity of the brain parenchymal lesions．Methods：Thirty cats were randomly divided into 2 groups，operation group(n=24)and sham operation group(n=6)．Operation group was performed by injection of the cellulose acetate polymer(CAP)solution into the superior sagittal sinus(SSS)to establish the acute cerebral venous occlusion，while sham operation group underwent only the exposure of SSS．MR1 was performed at l，3，6，12，24 and 48h after operation．Results：Twenty-two cats in operation group were operated successfully．Twenty-five lesions were detected on DWI in 14 cats．Thirty-one abnormal perfusion regions were detected o