高糖对大鼠肾成纤维细胞VEGF和PEDF表达的影响.pdfVIP

·274· 竖壁痘盘壹 98年6月第8卷第6期 J Clin Nephrol June 2008，Vo1．8，No．6 · 实验研 究 · 高糖对大鼠肾成纤维细胞VEGF和PEDF 表达的影响 李珍瑾 李竞 包艳 叶迎春 【摘要】 目的 探讨高糖对体外培养的大鼠肾成纤维细胞(NRK)血管内皮生长因子(vascular endothelial growth factor，VEGF)和色素上皮衍生因子(pigment epithelium derived factor，PEDF)表 达的影响。方法 采用RT-PCR分析方法，测定在不同浓度高糖、不同时间点NRK细胞Ⅶ GF与 PEDFmRNA的表达。设5．6 mmol／L葡萄糖组为正常对照组。结果 与正常组相比，加入不同浓度 高糖的各组NRK细胞VEGF rnRNA的表达明显升高(PO．05)，PEDF mRNA的表达明显下降(P d0．05)，呈剂量依赖性改变；随着各组作用时间的延长，VEGF mRNA的表达呈上升趋势(P 0．05)，PEDFmRNA的表达呈下降趋势(PO．05)，呈时间依赖性改变；VEGF与PEDF之间呈明显 负相关(r=一0．811，PO．05)。结论 在高糖环境中，体外培养的NRK细胞VEGF和PEDF表达 失衡，PEDF表达下降的同时VEGF表达增加，两者的表达失衡可能在糖尿病肾病(DN)的发生发展 中起一定的作用。 【关键词】 血管内皮生长因子；色素上皮衍生因子；高糖；成纤维细胞 Eff酏t 0f high glue~e on the expression of VEGF and PEDF in rat renal fibroblasts LI Zhen-jin．LJ Jing，BA0 Yah，et a1．Department of Endocrinology，Renmin Hospital，Wuhan University，Wuhan 430060，C ina [Abstract] 0bjective To explore the effect of high glucose on the expression of vascular endo— thelial growth factor(VEGF)and pigment epithelium derived factor(PEDF)in the cultured rat renal fibroblasts(NRK cells)．Methods NRK cells were cultured in vitro，and then divided into 5 groups： normal concentration glucose group(group N，5．6 mmol／L D-glucose)，mannitol group(group M， 5．6 mmol／L D-glucose+25 mmol／L mannito1)，high concentration glucose(HG)group(group A，10 mmol／L D-glucose；group B，20 mmol／L D-glucose；group C，30 mmol／L D-glucose)．The expres— sion levels of VEGF and PEDF mRNA were detected by using RT—PCR method．Results As compare with group N．the expression level of VEGF rnRNA was remarkably increased and that of PEDF mR— NA remarkably decreased in a dose and timedependent manner when the NRK cells were exposed to different high concentrations of glucose for different durations(P％0．05)．There was a significant negative correlation between PEDF 1evels and VEGF 1evels in NRK cells(r=一O．811，PO．O