高铜对雏鸭脾影响的组织病理学观察.pdfVIP

中国兽医科学 2008，38(07)：61 6-621 Chinese Veterinary Science 中图分类号：S 81 6．72 文献标识码：A 文章编号：1 673—4696(2008)07—061 6-06 高铜对雏鸭脾影响的组织病理学观察 杨 帆，崔恒敏 ，赵 丽，彭 西，邓俊良 (四川农业大学 动物医学院，四川 雅安 625014) 摘要：选用1日龄天府肉鸭360只，随机分为6组，分别以对照日粮(Cu 8 mg／kg)和高铜日粮(I组：Cu 100 mg／kg，II组：Cu 200 mg／kg，II组：Cu 400 mg／kg，IV组：Cu 600 mg／kg，V组：Cu 800 mg／kg)饲喂6周， 对高铜引起的雏鸭脾的病理损伤进行了观察。结果显示，I、Ⅱ组雏鸭的脾组织学变化与对照组比较差别不 明显，Ⅲ、Ⅳ、V组脾出现不同程度的淋巴细胞减少，电镜下，Ⅲ、Ⅳ、V组脾淋巴细胞线粒体肿大，嵴断裂甚至 消失，核染色质积聚于核膜下呈环形或团块状；II、IV、V组脾的绝对重量、生长指数显著降低(P0．05或 PG0．01)；II、lV、V组脾淋巴细胞的静止期延长，增殖指数明显下降(P0．05或P0．01)；II、IV、V组脾 淋巴细胞的凋亡率显著高于对照组(P0．05或PG0．01)。证实，日粮铜含量400～800 mg／kg可不同程度 地抑制雏鸭脾的发育，导致雏鸭细胞免疫功能和体液免疫功能受损。 关键词：高铜；脾；病理变化；生长指数；细胞周期；雏鸭 Hist0path0l0gical observation on effect of high dietary copper on spleen in ducklings YANG Fan，CUI Heng—rain，ZHA()Li，PENG Xi，DENG jun—liang (College of Veterinary Medicine。SichuanAgricultural University，Ya’n625014，China) Abstract：To observe the pathological lesions caused by high copper on the duckling spleen，360 one— day—old meat—type Tianfu ducklings were divided randomly into control，and Groups I，Ⅱ，Ⅲ，IV and V， which were fed with diets supplemented with 8，100，200，400，600 and 800 mg Cu per kilogram for 6 weeks， respectively．Histological changes were observed in ducklings in Groups II，IV and V in comparison with those in the contro1．U1trastructurally，the mitochondria in these three groups were swelled，and their crista were broken and disappeared．The chromatins in Groups llI，IV and V were also condensed．The weight and the proliferating index of spleen were lower in Groups II，IV and V than those in the control group(PG0．05 Or P G 0．0 1)．The number of GO+G1 phases splenocytes was high，and the proliferating index was low in Groups II，IV and V compared with those in the control group(PG0．05 or PGO．01)．Percentages of apoptotic lymphocytes in spleen were higher in Gro