核因子κB活性及环氧化酶2在胃癌及胃炎病变中表达探究.docVIP

核因子κB活性及环氧化酶2在胃癌及胃炎病变中表达探究【摘要】 目的研究胃癌及慢性浅表性胃炎组织中核因子κB（NF-κB）的DNA结合活性，p65及环氧合酶2（COX-2）的表达，通过比较两者表达的差异，从而探讨NF-κB及 COX-2在胃癌病程中的意义。 方法收集我院65例外科手术胃癌及癌旁组织标本，58例消化内镜慢性浅表性胃炎钳夹组织标本。采用凝胶迁移滞留试验EMSA检测其NF-κB DNA结合活性，免疫组化SABC法测定其NF-κB p65及 COX-2蛋白表达水平。 结果胃癌标本NF-κB DNA结合活性更高，可见明显核移位现象，与癌旁组织及胃炎组织比较具有统计学差异（ P 0．01）,NF-κB p65在胃癌组织，癌旁组织及胃炎中表达阳性率分别为87．7%，10．8%和55．2%，COX-2在胃癌组织，癌旁组织及胃炎中表达阳性率分别为100%，12．3%和53．4%。相关性分析显示胃癌组织中NF-κB p65和COX-2表达呈明显正相关（r＝0．6， P 0．01）。 结论NF-κB和COX-2在胃癌组织中阳性表达显著高于癌旁组织和慢性浅表性胃炎组织，且两者存在明显正相关，推测可能通过干预其活性和表达对胃癌的发生发展过程研究有一定帮助。 【关键词】核因子κB;环氧化酶2；胃癌；慢性浅表性胃炎 Activity of Nuclear Factor-kappa B and the Expression of Cyclooxygenase-2 in Human Gastric Carcinoma and Chronic Superficial Gastritis YANG Ming-shi,CHEN Ken. Department of digestive disease,the affiliated hospital of Guangdong medicine college,Zhanjiang 524001,China 【Abstract】 ObjectiveStudy on the activity of Nuclear Factor-kappa B (NF-κB） DNA-binding and the expression of p65,Cyclooxygenase-2(COX-2) in human gastric carcinoma and Chronic Superficial Gastritis.To explore the significance of NF-κB and COX-2 in the development of gastric carcinoma. MethodsWe collected 65 samples of gastric carcinoma in surgery and 58 samples of chronic superficial gastritis with gastroscope.The activity of NF-κB DNA-binding was detected by electrophoretic mobility shift assay (EMSA).And the expression of NF-κB-p65 and COX-2 were determined by immunohistochemical analysis of formalin-fixed,paraffin-embeded. ResultsThe activity of NF-κB DNA-binding was higher in carcinoma tissue than in the other tissue by EMSA ( P 0．05).There is significant between these two groups（ P 0．05）.The positive expression proportion of NF-κB-p65 were 87．7%in carcinoma,10．8%in normal gastric mucosa and 55．2%in chronic superficial gastritis.The expression of COX-2 was detected in each group.It was 100%in gastric carcinoma,was significantly higher than those in normal gastric mucosa(12．3%) and chronic superficial gastritis (53．4%)（ P 0．01）. Conclus