黑皮素-1受体在增生性瘢痕组织中表达及分布.docVIP

黑皮素-1受体在增生性瘢痕组织中表达及分布[摘要]目的：研究黑皮素-1受体（melanocortin-1 receptor, MC-1R）在增生性瘢痕组织中的表达和分布特点，探讨它与增生性瘢痕形成的关系。方法：利用兔抗人MC-1R抗体进行免疫组织化学染色，并测定8例增生性瘢痕，8例表浅性瘢痕和16例同体的正常皮肤标本切片染色后的平均灰度值，分别检测MC-1R在上述不同组织中表达的差异。结果：在正常皮肤、表浅瘢痕和增生性瘢痕中，MC-1R免疫阳性产物呈棕黄色颗粒状，主要分布于表皮基底层细胞胞浆中。在增生性瘢痕组织中，MC-1R灰度值（159.2±5.5）明显高于正常皮肤（134.7±5.9）和表浅性瘢痕（135.9±9.3），差异显著（P＜0.01）。而后两者无明显差异(P＞0.05)。结论：增生性瘢痕的形成可能与 MC-1R在增生性瘢痕组织中表达明显减少，由其介导的α-黑色素细胞刺激素（α-melanocyte stimulating hormone,α-MSH）对胶原蛋白的代谢和细胞外基质沉积的调节作用减弱有关。 [关键词]瘢痕；增生性；黑皮素-1受体 [中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455（2007）09-1178-03 The study of expression and distribution of melanocortin-1 receptor in hypertrophic scar CHENG Yong, LONG Jian-hong （Department of Plastic and Burns Surgery,the First People’s Hospital of Chenzhou,Chenzhou 423000,Hunan,China) Abstract:Objective To observe the expression and the distribution of melanocortin-1 receptor (MC-1R) in hypertrophic scar and then identifying the relationship between MC-1R and formation of hypertrophic scar.MethodsBioptic samples were taken from 8 cases of hypertrophic scar, 8 cases of superficial scar and 16 cases of controlled normal self-skin.MC-1R immunohistochemistry staining and its grey scale were examined in each sample.ResultsIn normal skin, superficial scar and hypertrophic scar, MC-1R immune positive material was yellow-brown granule, mainly distributed in plasma in basal lamina of epidermal. semi quantitative diction showed that the mean of MC-1R grey scale in hypertrophic scar(159.2±5.5) was significantly higher than that of normal skin（134.7±5.9）and superficial scar（135.9±9.3）, respectively（P＜0.01）. But, there was no significant deference between normal skin and superficial scar. ConclusionOur result suggested that the formation of hypertrophic scar was possibly relative to the decrease in expression of MC-1R in hypertrophic scar and then the regulation of α-MSH by MC-1R in the sediment of extracellular matrix. Key words: cicatrix; hypertrophic; receptor melanocortin type 1 瘢痕是