cardiac gene activation analysis in mammalian non-myoblasic cells by nkx2-5, tbx5, gata4 and myocd在哺乳动物的心脏基因活化分析non-myoblasic nkx2-5细胞,tbx5,gata4 myocd.pdfVIP

Cardiac Gene Activation Analysis in Mammalian Non- Myoblasic Cells by Nkx2-5, Tbx5, Gata4 and Myocd 1 2 3 4 1 Lei Zhou *, Yu Liu , Li Lu , Xinzheng Lu , Richard A. F. Dixon * 1 Department of Molecular Cardiology, Texas Heart Institute, Houston, Texas, United States of America, 2 Department of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America, 3 Department of Biochemistry and Molecular Biology, University of Texas, M. D. Anderson Cancer Center, Houston, Texas, United States of America, 4 Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China Abstract Cardiac transcription factors are master regulators during heart development. Some were shown to transdifferentiate tail tip and cardiac fibroblasts into cardiomyocytes. However, recent studies have showed that controversies exist. Potential difference in tail tip and cardiac fibroblast isolation may possibly confound the observations. Moreover, due to the use of a cardiac reporter (Myh6) selection strategy for induced cardiomyocyte enrichment, and the lack of tracking signals for each transcription factors, individual roles of each transcription factors in activating cardiac gene expression in mammalian non- myoblastic cells have never been elucidated. Answers to these questions are an important step toward cardiomyocyte regeneration. Because mouse 10T1/2 fibroblasts are non-myoblastic in nature and can be induced to express genes of all three types of muscle cells, they are an ideal model for the analysis of cardiac and non-cardiac gene activation after induction. We constructed bi-cistronic lentiviral vectors, capable of expressing cardiac transcription factors along with different