coral fluorescent proteins as antioxidants珊瑚荧光蛋白作为抗氧化剂.pdfVIP

Coral Fluorescent Proteins as Antioxidants 1,2 3 4 Caroline V. Palmer *, Chintan K. Modi , Laura D. Mydlarz 1 School of Biology, Newcastle University, Newcastle upon Tyne, United Kingdom, 2 ARC Centre of Excellence for Coral Reef Studies and School of Marine and Tropical Biology, James Cook University, Townsville, Queensland, Australia, 3 Integrative Biology, University of Texas at Austin, Austin, Texas, United States of America, 4 Department of Biology, University of Texas at Arlington, Arlington, Texas, United States of America Abstract Background: A wide array of fluorescent proteins (FP) is present in anthozoans, although their biochemical characteristics and function in host tissue remain to be determined. Upregulation of FP’s frequently occurs in injured or compromised coral tissue, suggesting a potential role of coral FPs in host stress responses. Methodology/Principal Findings: The presence of FPs was determined and quantified for a subsample of seven healthy Caribbean coral species using spectral emission analysis of tissue extracts. FP concentration was correlated with the in vivo antioxidant potential of the tissue extracts by quantifying the hydrogen peroxide (H O ) scavenging rates. FPs of the seven 2 2 species varied in both type and abundance and demonstrated a positive correlation between H2O2 scavenging rate and FP concentration. To validate this data, the H2O2 scavenging rates of four pure scleractinian FPs, cyan (CFP), green (GFP), red (RFP) and chromoprotein (CP), and their mutant counterparts (without chromophores), were investigated. In vitro, each FP scavenged H2O2 with the most efficient being CP followed by equivalent activi