the ppar ligand gw501516 reduces growth but not apoptosis in mouse inner medullary collecting duct cellsppar配体gw501516减少了增长,但不会在老鼠内髓集合管细胞凋亡.pdfVIP

Hindawi Publishing Corporation PPAR Research Volume 2009, Article ID 706283, 11 pages doi:10.1155/2009/706283 Research Article The PPARδ Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells ´ Jordan Clark, Rania Nasrallah, and Richard L. Hebert Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada K1H 8M5 ´ Correspondence should be addressed to Richard L. Hebert, rlhebert@uottawa.ca Received 26 August 2008; Revised 12 December 2008; Accepted 5 January 2009 Recommended by Xiong Ruan The collecting duct (CD) expresses considerable amounts of PPARδ. While its role is unknown in the CD, in other renal cells it has been shown to regulate both growth and apoptosis. We thus hypothesized that PPARδ reduces apoptotic responses and stimulates cell growth in the mouse CD, and examined the effect of GW501516, a synthetic PPARδ ligand, on these responses in mouse IMCD-K2 cells. High doses of GW501516 decreased both DNA and protein synthesis in these cells by 80%, but had no overall effect on cell viability. Although anisomycin treatment resulted in an increase of caspase-3 levels of about 2.59-fold of control, GW501516 did not affect anisomycin-induced changes in active caspase-3 levels. These results show that a PPARδ ligand inhibits growth but does not affect anisomycin-apoptosis in a mouse IMCD cell line. This could have therapeutic implications for renal diseases associated with increased CD growth responses. Copyright © 2009 Jordan Clark et al. This is an open access article distributed under the Creative Commons Attribut