genistein to promote breast cancer cell proliferation and igf 1r mrna expression mechanism（染料木黄酮促进乳腺癌细胞增殖和igf 1 r mrna表达机制）.docVIP

下载本文档

3
0
约1.22万字
约 12页
2017-09-06 发布于浙江
举报
版权申诉

genistein to promote breast cancer cell proliferation and igf 1r mrna expression mechanism（染料木黄酮促进乳腺癌细胞增殖和igf 1 r mrna表达机制）.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

genistein to promote breast cancer cell proliferation and igf 1r mrna expression mechanism（染料木黄酮促进乳腺癌细胞增殖和igf 1 r mrna表达机制）

Genistein to promote breast cancer cell proliferation and IGF 1R mRNA expression mechanism [Abstract] Objective genistein promote human breast cancer (MCF 7 cell proliferation and IGF 1R mRNA expression of mechanisms. Method in MCF 7 cells in conventional culture, with ICI182, 780 or JB 1 and genistein co-cultured in order to block the estrogen receptor (ER or IGF 1R.MTT method, cell growth was observed by RT  PCR technique to observe IGF 1R gene expression results genistein in MCF 7 cells with proliferative effect, compared with the control group difference was statistically significant (F = 14.627, q = 3.76, P lt;0.05), this effect can be ER antagonist ICI182, 780 or IGF 1R antagonist JB 1 was completely blocked (q = 3.89,4.25, P lt;0.05), and genistein increase IGF 1R gene expression (F = 21.695, q = 4.94, P lt;0.01, this effect can be ICI182, 780 or JB  1 was completely blocked (q = 5.03,5.89, P lt;0.01. conclusion genistein can promote MCF  7 cell proliferation and IGF 1R mRNA expression, this effect can be ER antagonist ICI182, 780 or IGF 1R antagonist JB completely blocked. [Keywords:] genistein; receptor, IGF1-receptor, estrogen; breast tumor [ABSTRACT] ObjectiveTo study the mechanism of stimulating effects of genistein on breast cancer cell proliferation and IGF 1R mRNA expression. MethodsMCF 7 cells were cultured in genistein with or without ICI182, 780 and JB1, respectively. ICI182, 780 was used to block the estrogen receptor (ER) and JB 1 to block the insulin like growth factor 1 receptor (IGF 1R). The cell proliferation was observed via MTT method. The IGF 1R mRNA expression was detected by RT PCR.ResultsGenistein stimulated the growth of MCF 7 cells ( F = 14.627, q = 3.76, P lt;0.05), which could be completely blocked by ER anta gonist ICI182, 780 or IGF 1R antagonist JB 1 (q = 3.89,4.25; P lt;0.05). Genistein increased the IGF 1R gene expression (F = 21.695, q = 4.94, P lt;0.01) and this effect could also be entirely blocked by ICI182,