high-density fermentation about oriented of interferon α2a engineering bacteria（高密度发酵的面向干扰素α2a工程细菌）.docVIP

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high-density fermentation about oriented of interferon α2a engineering bacteria（高密度发酵的面向干扰素α2a工程细菌）.doc

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high-density fermentation about oriented of interferon α2a engineering bacteria（高密度发酵的面向干扰素α2a工程细菌）

High-density fermentation about oriented of interferon α2a engineering bacteria Papers to write network: : Meng Jie such, YAN Zhen, Zhao Ning, Zhang Ying [Keywords] E. coli [Abstract] AIM: To optimize the pilotscale fermentation procedure of E. coli producing tumor targeted human of IFN @ 2a and to obtain high cell density and high level expression of the by rang of the aim protein. METHODS: The effects of pH, dissolved oxygen, culture time, induction time and the manner of feeding were analyzed with 10 L of fermentation tank. RESULTS: Under the established conditions, we performed a threetime repeated fermentation. The final cell density was A600 nm 25-30 and more than 50 g of wet bacteria per liter could be obtained. The expression level of recombinant protein was higher than 3.2 ?109 U / L. CONCLUSION: The results show that the established fermentation procedure is stable, of short cycle and with high expression, which lays the basis for further purification and largescale production of IFN @ 2aNGR. [Keywords] fermentation; IFN; Escherichia.coli [Abstract] Objective: To optimize the pilot fermentation process to get the engineered bacteria E. coli expression-oriented human interferon @ 2a high density fermentation and purpose protein expression. Methods: fermentor, on the impact of engineering bacteria growth and target protein expression in conditions, such as pH value, activation time, induction time, dissolved oxygen range and complement stream plus optimize Results: Under the optimized conditions, consecutive batches Duplicate 10 L fermentation engineering bacteria, the final cell density. reached A600 nm 25 ~ 30, the bacteria get volume up to wet weight of 50 g / L or more, the amount of target protein expression are more than 3.2 ?109 U / L Conclusion: This fermentation process with a short cycle, high yield and repeatability good stability and lay the foundation for downstream purification and further large-scale production. [