七氟烷对老年大鼠认知功能和海马CA1区IGF-ⅠmRNA表达地影响.pdfVIP

徐州医学院硕士学位论文 The effect of sevoflurane anesthesia on cognitive functions in gerontal rats and the expression of insulin-like growth factor- ⅠmRNA in CA1 region of hippocampus Abstract Object To study the effect of sevoflurane anesthesia on cognitive functions in gerontal Sprague-Dawley rats and the expression of insulin-like growth factor- ⅠmRNA in CA1 region of hippocampus. Methods Experiments were performed on 40 twelve-months-old SD rats of either sex. They were randomly assigned to 5 groups(n = 8/group): the normal control group （group A ）; group B and group C inhaled 1.5 % sevoflurane 2h, group D and group E inhaled 3.0 % sevoflurane 2h, group B and group D be tested after received sevoflurone 1 day, group C and group E be tested after received 3.0 % sevoflurone 7 day. All rats received 1.5 % or 3.0 % sevoflurone in a special glass anesthesia box 2 hours respectively excepct the normal control. Y-maze was used to test the ability of learning and memory on rats after received sevoflurone 1 or 7 days at the same moment portion. Hippocampus exemplares of rats were immediately took out after the tests. The hippocampus was isolated for determination of IGF- Ⅰ mRNA expression using RT-PCR technique. Results In D group, 3.0 % sevoflurane induced cogntive decline and decreased IGF- Ⅰ mRNA expression significantly at 1 d. But there was no significant difference between the other groups. Conclusion Sevoflurane anesthesia can induce temporary cogntive decline and decreased IGF- ⅠmRNA expression in hippocampus CA-1 region in gerontal rats, 3 徐州医学院硕士学位论文 suggesting the endogenous IGF- Ⅰ expression levels declined may contribute to these phenomenons. Key words sevoflu