serum ige reactivity profiling in an asthma affected cohort血清ige反应分析哮喘影响队列.pdfVIP

Serum IgE Reactivity Profiling in an Asthma Affected Cohort 1. 2. 1 3 4 Tania Dottorini , Gabriella Sole , Luisa Nunziangeli , Francesca Baldracchini , Nicola Senin , Giorgio 3 1 2 1,5 Mazzoleni , Carla Proietti , Lenuta Balaci , Andrea Crisanti * 1 Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy, 2 National Research Council of Italy (CNR), Institute of Genetics and Biomedical Research (IRGB), Cagliari, Italy, 3 Microtest Matrices LtD, London, United Kingdom, 4 Department of Industrial Engineering, University of Perugia, Perugia, Italy, 5 Biological Sciences, Imperial College London, London, United Kingdom Abstract Background: Epidemiological evidence indicates that atopic asthma correlates with high serum IgE levels though the contribution of allergen specific IgE to the pathogenesis and the severity of the disease is still unclear. Methods: We developed a microarray immunoassay containing 103 allergens to study the IgE reactivity profiles of 485 asthmatic and 342 non-asthmatic individuals belonging to families whose members have a documented history of asthma and atopy. We employed k-means clustering, to investigate whether a particular IgE reactivity profile correlated with asthma and other atopic conditions such as rhinitis, conjunctivitis and eczema. Results: Both case-control and parent-to-siblings analyses demonstrated that while the presence of specific IgE against individual allergens correlated poorly with pathological conditions, particular reactivity profiles were significantly associated with asthma (