serum microrna-21 as marker for necroinflammation in hepatitis c patients with and without hepatocellular carcinoma丙型肝炎病人血清microrna-21标记为坏死性炎症和肝细胞癌.pdfVIP

Serum MicroRNA-21 as Marker for Necroinflammation in Hepatitis C Patients with and without Hepatocellular Carcinoma 1. 1. 1 1 1 ¨ Verena Bihrer , Oliver Waidmann , Mireen Friedrich-Rust , Nicole Forestier , Simone Susser , Jorg 1¤ 1 1 1 1 1 Haupenthal , Martin Welker , Ying Shi , Jan Peveling-Oberhag , Andreas Polta , Michael von Wagner , 2 1 ¨ 1 1 1 Heinfried H. Radeke , Christoph Sarrazin , Jorg Trojan , Stefan Zeuzem , Bernd Kronenberger , Albrecht Piiper1* 1 Department of Medicine I, University of Frankfurt/M., Frankfurt, Germany, 2 Institute of Pharmacology/ZAFES, University of Frankfurt/M., Frankfurt, Germany Abstract Background: MicroRNA-21 (miR-21) is up-regulated in tumor tissue of patients with malignant diseases, including hepatocellular carcinoma (HCC). Elevated concentrations of miR-21 have also been found in sera or plasma from patients with malignancies, rendering it an interesting candidate as serum/plasma marker for malignancies. Here we correlated serum miR-21 levels with clinical parameters in patients with different stages of chronic hepatitis C virus infection (CHC) and CHC-associated HCC. Methodology/Principal Findings: 62 CHC patients, 29 patients with CHC and HCC and 19 healthy controls were prospectively enrolled. RNA was extracted from the sera and