severe hemorrhagic fever in strain 13n guinea pigs infected with lujo virus严重的出血热应变13 n豚鼠lujo病毒感染.pdfVIP

Severe Hemorrhagic Fever in Strain 13/N Guinea Pigs Infected with Lujo Virus 1 1,2 1 ´ ˜ 1 1 Brian H. Bird *, Kimberly A. Dodd , Bobbie R. Erickson , Cesar G. Albarino , Ayan K. Chakrabarti , 1 1 ¨ 1 1 1 Laura K. McMullan , Eric Bergeron , Ute Stroeher , Deborah Cannon , Brock Martin , JoAnn D. Coleman- 1 1 1 McCray , Stuart T. Nichol , Christina F. Spiropoulou * 1Viral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America, 2 School of Veterinary Medicine, University of California Davis, Davis, California, United States of America Abstract Lujo virus (LUJV) is a novel member of the Arenaviridae family that was first identified in 2008 after an outbreak of severe hemorrhagic fever (HF). In what was a small but rapidly progressing outbreak, this previously unknown virus was transmitted from the critically ill index patient to 4 attending healthcare workers. Four persons died during this outbreak, for a total case fatality of 80% (4/5). The suspected rodent source of the initial exposure to LUJV remains a mystery. Because of the ease of transmission, high case fatality, and novel nature of LUJV, we sought to establish an animal model of LUJV HF. Initial attempts in mice failed, but infection of inbred strain 13/N guinea pigs resulted in lethal disease. A total of 41 adult strain 13/N guinea pigs were infected with either wild-type LUJV or a full-length recombinant LUJV. Results demonstrated