原创力文档aav-mediated human pedf inhibits tumor growth and metastasis in murine colorectal peritoneal carcinomatosis modelaav-mediated人类pedf抑制肿瘤生长和转移的小鼠结肠直肠腹膜癌扩散模型.pdfVIP
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aav-mediated human pedf inhibits tumor growth and metastasis in murine colorectal peritoneal carcinomatosis modelaav-mediated人类pedf抑制肿瘤生长和转移的小鼠结肠直肠腹膜癌扩散模型
Wu et al. BMC Cancer 2012, 12:129
/1471-2407/12/129
RESEARCH ARTICLE Open Access
AAV-mediated human PEDF inhibits tumor
growth and metastasis in murine colorectal
peritoneal carcinomatosis model
1† 1† 1 1 1 1 1
Qin Jie Wu , Chang Yang Gong , Shun Tao Luo , Dong Mei Zhang , Shuang Zhang , Hua Shan Shi , Lian Lu ,
1 1 1 1* 2 1
Heng Xiu Yan , Sha Sha He , Dan Dan Li , Li Yang , Xia Zhao and Yu Quan Wei
Abstract
Background: Angiogenesis plays an important role in tumor growth and metastasis, therefore antiangiogenic
therapy was widely investigated as a promising approach for cancer therapy. Recently, pigment epithelium-derived
factor (PEDF) has been shown to be the most potent inhibitor of angiogenesis. Adeno-associated virus (AAV)
vectors have been intensively studied due to their wide tropisms, nonpathogenicity, and long-term transgene
expression in vivo. The objective of this work was to evaluate the ability of AAV-mediated human PEDF (hPEDF) as
a potent tumor suppressor and a potential candidate for cancer gene therapy.
Methods: Recombinant AAV encoding hPEDF (rAAV -hPEDF) was constructed and produced, and then was
2 2
assigned for in vitro and in vivo experiments. Conditioned medium from cells infected with rAAV2-hPEDF was used
for cell proliferation and tube formation tests of human umbilical vein endothelial cells (HUVECs). Subsequently,
colorectal peritoneal carcinomatosis (CRPC) mouse model was established and treated with rAAV2-hPEDF.
Therapeutic efficacy of rAAV2-hPEDF were investigated, includin
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