nuclear protein ik undergoes dynamic subcellular translocation and forms unique nuclear bodies during the cell cycle核蛋白质动力学进行动态亚细胞易位和形式独特的核体在细胞周期.pdfVIP

Hu et al. Biomarker Research 2013, 1:11 /content/1/1/11 RESEARCH Open Access Nuclear protein IK undergoes dynamic subcellular translocation and forms unique nuclear bodies during the cell cycle 1 1 1 1 1,2* Liyan Hu , Feikun Yang , Xianan Liu , Dazhong Xu and Wei Dai Abstract IK is a nuclear protein containing a unique domain named RED due to the presence of a repetitive arginine (R), aspartic (E), and glutamic acid (D) sequence. To date, the function of this protein remains largely unknown despite of a couple of previous studies in the literature. Here we report that depletion of IK via RNA interference results in mitotic arrest. We also demonstrate that IK undergoes dynamic translocation during interphase and mitosis. In particular, IK is primarily present in some interphase cells as nuclear foci/bodies which do not co-localize with nucleoli, PMA bodies and Cajal bodies. Pull-down analysis coupled with mass spectrometry reveals that IK is associated with DHX15, a putative ATP-dependent RNA helicase. Our results strongly suggest that IK may participate in pre-mRNA splicing and that it may be a useful biomarker for a new nuclear structure in the cell. Introduction spectrometry analyses reveal that IK interacts with IK was originally identified as a cytokine that inhibits DHX15, a putative ATP-dependent RNA helicase which interferon gamma (IFN-γ)-induced expression of HLA is implicated in pre-mRNA splicing. Our current study class II antigen during immune responses in K562 suggests that IK can be explored as a new biomarker for erythroleukemic cell line [1]. The protein got its