斑马鱼胚胎发育技术诊断百草枯生物毒性的研究.pdf

斑马鱼胚胎发育技术诊断百草枯生物毒性的研究.pdf

关文摘要 3To the studyinteractionof withDNA. paraquat 4Toobservetheeffects of tozebrafish paraquat embryo and theeffects tolifein investigate water. ofparaquat Methods: 1 Putthe intothe sample mixed ofmethanoland liquor acid and hydrochloric0H4—5)one extract night through ultrasonic extractthe withC1 sound,then liquor 8tubeand the of in withion analyzequantity paraquatplants pair reversed-phaseliquid mobile is chromatography.The phase sodium phosphatebuffer(O.18mol/L)with (18mm01)and with methylCyanides(9:1),then adjustpH=2 is solution diethylamine.The wavelength 256nm.Dropparaquat and1 and the into (10.0l_tg/ml 00.0I-tg/m1)inspinach put spinach and natural themetabolism fridge environment,observe conditionof in aider and paraquat spina

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