L-NAME对隐睾下降固定术后生精细胞凋亡影响.docx

normal saline and L-NAME(50mg/Kg) accordingly. The blood samples were collectedfrom the tail after injection two hours on the 12th day.The serum were collected andstored at –80℃ for subsequent detection. Serum NO concentration was measured byGreiss method . The activity of NOS in serum was measured by colorimetric method.The rats were sacrificed on the 24th day after the second operation. The morphology ofspermatogenic epithelium in rats of every group was observed with light microscopy.The level of spermatogenic cell apoptosis in the unilateral cryptorchidism andorchidopexy rats were evaluated by FCM and TUNEL.The protein expression level ofeNOS and iNOS in testes were detected by Western blot and immunohistochemicalassay accordingly.Results: The level of spermatogenic cell apoptosis, serum NO concentration andthe activity of NOS in rats cryptorchidism were higher than the rats orchidopexy but theweight of testes were lighter than the rats orchidopexy. Administration of L-NAMEcould decrease serum NO concentration and spermatogenic cell apoptosis in ratsexperimental orchidopexy, and it could significantly inhibit weight lose of theorchidopexied testes. The activity of NOS was attenuated significantly by L-NAME.The protein expression level of eNOS and iNOS in the testes of the sham operationgroup was the lowest in the four groups. The expression ofeNOS and iNOS in thespermatogenic epithelium were feeble in the sham operation group but strong in otherthree groups by immunohistochemical assay.8Conclusions: 1. The improvement ofspermatogenesis is relate to the reduction ofspermatogenic cell apoptosis in rats experimental orchidopexy.2. NO is the important biology active gene of excess apoptosis inspermatogenic cell in rats experimental orchidopexy.3. L-NAME can reduce the production of NO and spermatogenic cellapoptosis,it shows that the inhibitor of NOS can promote the enhancement ofspermatogenic capability in rats experimental orchidopexy.Postgraduate: Shi Jinfeng(Hum