ox-LDL对骨髓干细胞分化平滑肌细胞泡沫化影响.docxVIP

total cholesterol and cholesterol ester in BMSC-SMC increased gradually with timeprolonged, and the contents of total cholesterol reached 304.3±26.4μg/mg in 72h,compared with untreated cells 28.8±3.6μg/mg. which raised 9.5times（P〈0.05）；the ratioof cholesterol ester to total cholesterol was 70.1% and raised 2.2times（P〈0.05），whichcorrespond to the characteristic of foam pared with that of VSMC treated with thesame method. After treated by ox-LDL with 80mg/L for 72h，the contents of totalcholesterol reached 215.7.3±18.9μg/mg，the ratio of cholesterol ester to total cholesterol inVSMC was 60.1%. compared with BMSC-SMC, variance is significance(P0.05)After treated by ox-LDL with 0、20、40、80mg/L for 72h, the contents of totalcholesterol in BMSC-SMC reached29.4±2.2、137.3±7.3、171.3±11.7、295.9±20.3μg/mg;the ratio of cholesterol ester to total cholesterol was 23.6%、38.4%、48.8%、68.5%，suggestincreased gradually with concentration of ox-LDL , Cellular totol cholesteroland andEster cholesterol also increased gradually.After treated by ox-LDL with 80mg/L for 72h, Oil Red O staining showed that thevolume of BMSC-SMC was augmentation and a lot of lipid droplet appeared in cells,which was the typical form of foam cells. Lipid droplet in cells was significantly increasedcompared with that of VSMC.3、Western blotting analysis showed that the expression of CD36 was low inBSMC-MSC. After treated by ox-LDL with 80mg/L for 72h, the expression of CD36 wassignificantly increased,the same treated in VSMC the expression of CD36 was increasedtoo, but the extent of increasion compared with that of VSMC is weak（P〈0.05）.Conclusion 1、Compared with VSMC that isolated from Aortic tunica media,BMSC-SMC can accumulate a lot of lipid droplet and form foam cells after treated byox-LDL, suggested that BMSC-SMC maybe participate and facilitate the occurrence anddevelopment of artherosclerosis(As)2、The accumulation of cholesterol ester and foam in BVSMC after treated by ox-LDL isrelate with the up-regulation of