微波双酶协同水解大豆分离蛋白制备小分子肽的研究 STUDY ON MICROWAVE TO THE HYDROLYSIS SOYBEAN PROTEIN INTO LOW MOLECULAR WEIGHT PEPTIDES WITH DOUBLE ENZYMES COOPERATION.pdfVIP

科学研究 食品臻究与羿发2DD6．均／．27~0．8 9 微波双酶协同水解大豆分离蛋白 制备小分子肽的研究 刘静。2。陈均志1。+ (1．陕西科技大学化工学院，陕西咸阳712081；2．咸阳师范学院化学系，陕西咸阳712000) 摘要：研究微波加热条件下，用碱性蛋白酶和胰蛋白酶双酶水解大豆分离蛋白。以氨基氮为评价指标，确定了单酶 水解工艺，双酶分步水解的顺序和制备小分子大豆多肽的最佳条件，并通过毛细管电泳方法对水解多肽的分子量进 行测定。实验表明：双酶水解优于单酶，制得的大豆肽分子量主要集中在6000以下。 关键词：大豆分离蛋白；大豆多肽；双酶水解；微波；高效毛细管电泳 S7I’UDY0NMICROWAVE’110’IHEHYDROLYSISSOYBEANPR0’11EININ’lULUWMULECULAR WEIGHTPEPTIDESWITHDOUBLEENZYMES’C00PERATION 1’+ LIU Jun—zhi Jin91一，CHEN (1．School ofScienceand ofChemistryandEn西neer，Shaall】【iUniversity of 712000，Shaanxi，China) 2．DepaItnlentChemistry，XianyangTeacherS’CoUege，Xianyang to isolate Abstract：Underthemicrowave and wereused heating，alklingproteasetrypsin hydrolyzesoyprotein ofone orderdoubleen— intllis ANas conditions of standards，the hydrolysis，the p印er．Using optimized enzyme and conditionoflow haVebeen elec— mebest molecular found，with zymeshydrolysis wei曲tp01ypeptides c印illary of weredetennined．Theresultsshowsthatdouble molecular trophoresis，thewei曲tsoybean enzymes polypeptides is tot}la