响应曲面优化甘蔗糖蜜酸水解工艺研究 Optimization of Acid Hydrolysis Process of Sugarcane Molasses by Response Surface Method.pdfVIP

甘蔗糖业 2013 年第 5 期，2013 年 10 月 Sugarcane and Canesugar, No. 5, Oct. 2013 响应曲面优化甘蔗糖蜜酸水解工艺研究 1 2 1 1 1 1* 1 覃香香 ，凌启泉 ，周玉恒 ，蔡爱华 ，陈海珊 ，张厚瑞 ，沈育伊 1 2 （广西壮族自治区中国科学院广西植物研究所 广西植物功能物质研究与利用重点实验室，广西桂林 541006 ； 桂林 理工大学，广西桂林 541006 ） 摘 要：采用响应曲面法对影响甘蔗糖蜜水解总还原糖得率的 3 个主要影响因素即硫酸浓度、水解温 度、水解时间进行优化。利用 Design Expert 软件分析表明：在甘蔗糖蜜糖度 65、硫酸浓度 4% 、水解 温度 94 ℃、水解时间 66 min 的工艺条件下，100 g 甘蔗原糖蜜水解后获得总还原糖 54.98 g ，实测结果 与响应面拟合所得方程的预测值相符合。 关键词：甘蔗糖蜜；还原糖；硫酸水解；响应曲面法 中图分类号：TS249.3 文献标识码：A 文章编号：1005-9695(2013)05-0020-06 Optimization of Acid Hydrolysis Process of Sugarcane Molasses by Response Surface Method 1 2 1 1 1 1 QIN Xiang-xiang , LING Qi-quan , ZHOU Yu-heng , CAI Ai-hua , CHEN Hai-shan , ZHANG Hou-rui , SHEN Yu-yi 1 ( Guangxi Institute of Botany, Chinese Academy of Sciences/Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guilin, Guangxi 541006; 2Guilin University of Technology, Guilin, Guangxi 541006) Abstract: Three key factors affecting total reducing sugars yield from sugarcane molasses hydrolysis, such as sulfuric acid concentration, hydrolysis temperature, hydrolysis time, were optimized by response surface method. Design Expert analysis indicated that 55.9±0.2 g total reducing sugars can be obtained from hydrolyzing 100 g original sugar molasses under the conditions of sugar molasses Brix 65, sulfuric acid concentration 4%, hydrolysis temperature 94 ℃ and hydrolysis time 66 min. The actual result was consistent