中药虎杖水煎液对衰老小鼠学习、记忆功能的影响及机制 胡懿淋.docVIP

精品论文 参考文献 中药虎杖水煎液对衰老小鼠学习、记忆功能的影响及机制 胡懿淋 胡懿淋 (南通大学临床医学院 226001) 　　【摘要】目的 观察虎杖水煎液对D-半乳糖（D-gal）所致衰老小鼠学习、记忆功能的影响，并初步探讨其作用机制。方法 取ICR小鼠80只，雌雄兼用，随机分成4组：正常对照组（NG，n=20），模型对照组（MG，n=20），虎杖水煎液低剂量组（LG，5g/kg，n=20），虎杖水煎液高剂量组（HG，10g/kg，n=20）。正常对照组给予背部皮下注射生理盐水，其余各组用 D-gal按150mg/kg背部皮下注射6周，每日一次，造成亚急性衰老模型。造模同时，虎杖水煎液低剂量组和高剂量组分别给予相应浓度的虎杖水煎液，正常对照组和模型对照组给予相同容积的0.5% 羧甲基纤维素钠（CMC）。采用Y型电迷宫检测学习、记忆功能；使用相关试剂盒测定脑皮层中超氧化物歧化酶（SOD）和单胺氧化酶-B（MAO-B）的活力及丙二醛（MDA）和脂褐质（LP）的含量。结果 给予D-gal 6周后，Y迷宫检测模型组记忆功能明显下降，表现为不同时间的正确反应率降低，潜伏期延长；而虎杖水煎液能明显缩短衰老小鼠潜伏期，显著升高正确反应率。生化检测指标显示，模型组脑皮层中MDA和LP含量升高；虎杖水煎液能降低脑皮层中MAO-B活性，升高SOD活性，降低脑皮层中MDA和LP含量。结论 虎杖水煎液对D-gal诱导衰老模型小鼠的记忆功能有保护作用，其机制可能与其减少脑中MDA及LP含量，降低MAO-B活性，升高SOD活性有关。 　　【关键词】虎杖水煎液 衰老 学习 记忆 Y迷宫 小鼠 　　【中图分类号】R965 【文献标识码】A 【文章编号】1672-5085（2013）41-0031-02 　　【Abstract】 Aim: To investigate the effect of Rhizoma Polygoni Cuspidati (RPC) decoction on learning and memory function of aging model mice induced by D-galactose, and explore its mechanism preliminarily. Methods: 80 ICR mice, male and female were both used, were randomly divided into 4 groups: Normal control group (NG, n=20), Model control group (MG，n=20), Low-dose drug treatment group (LG，5g/kg，n=20), High-dose drug treatment group (HG，10g/kg，n=20).Except NG was subeutaneously injected with saline, other groups were given subcutaneously injection of D-galactose (D-gal) at 150 mg/kg once a day for six weeks to induce subacute aging model. At the same time LG was orally administrated RPC decoction at dose 5g/kg and HG at 10g/kg. NG and MG were given orally the same volume 0.5% carboxymethyl cellulose sodium(CMC). Y-maze was use to determine the function of learning and memory. The analytic kit was used to measure the content of malondialdehyde (MDA) and lipofuscin (LP), the activity of superoxide dismutase(SOD) and monoamine oxidase-B (MAO-B) in brain. Results: In Y-maze test, the correct response rate declined and the latency of first correct response has e