子痫前期患者血清和羊水Th1Th2细胞因子表达的研究.docVIP

精品论文 参考文献 子痫前期患者血清和羊水Th1Th2细胞因子表达的研究 俞奇(通讯作者) 蔡春芳(广东省中山市博爱医院妇产科 528400) 【摘要】目的 比较非孕妇女、正常妊娠孕妇及子痫前期患者外周血中白介素- 1beta;(IL- 1beta;) 、肿瘤坏死因子(TNF-alpha;)、IL-10和IL-4的水平, 探讨辅助T细胞（Th）1/ Th2 细胞因子在子痫前期中的作用。方法 产前子痫前期组45例(轻度子痫前期25 例, 重度子痫前期20例), 正常妊娠组20例, 非孕对照组10例。产前抽取研究对象静脉血3ml,离心后取血清保存于-80℃； 产程中取无菌羊水5ml，保存于-80℃。采用ELISA 法测定IL- 1beta;、TNF-alpha;、IL-10和IL-4的水平。结果 正常妊娠组血清和羊水中TNF-alpha;和IL-1beta;较非孕对照组有所降低，均Plt;0.05；而轻度子痫组孕妇血清和羊水的TNF-alpha;和IL-1beta;明显高于正常妊娠组孕妇，均Plt;0.05；重度子痫组孕妇血清和羊水的TNF-alpha;和IL-1beta;明显高于轻度子痫组，均Plt;0.05。正常妊娠组血清和羊水中IL-10和IL-4较非孕对照组有所升高，均Plt;0.05；而轻度子痫组孕妇血清和羊水的IL-10和IL-4明显低于正常妊娠组孕妇，均Plt;0.05；重度子痫组孕妇血清和羊水的IL-10和IL-4明显低于轻度子痫组，均Plt;0.05。 结论 Th1/Th2 型细胞因子的网络调节参与维持正常妊娠的进行,Th1型细胞因子的过度表达及免疫细胞的活化可致子痫前期妊娠。 【关键词】子痫前期 Th1 Th2 【中图分类号】R714.24+5【文献标识码】A【文章编号】2095-1752（2013）03-0138-02 【Abstract】Objective To study the role of the expression of interleukin-1beta; ( IL- 1beta;), tumor necrosis factor alpha (TNF-#61537;) , IL-10 and IL-4 in pre-eclampsia and explore the function of Th1/Th2 in pre-eclamptic patients. Methods IL- 1beta;,TNF-#61537;, IL-10 and IL-4 were measured by ELISA in 20 severe pre-eclamptic patients, 25 mild pre-eclamptic patients, 20 normal pregnant women and 10 non-pregnant women. Results Amniotic fluid and plasma IL- 1beta; and TNF-#61537; levels in normal pregnant women were reduced in non-pregnant women. But the levels of IL- 1beta; and TNF-#61537; in mild pre-eclamptic patients were higher than in normal pregnant women and lower than in severe pre-eclamptic patients. (all p lt;0.05). Amniotic fluid and plasma IL-10 and IL-4 levels in normal pregnant women were increased in non-pregnant women. But the levels of IL-10 and IL-4 in mild pre-eclamptic patients were reduced in normal pregnant women and higher than in severe pre-eclamptic patients. (all p lt;0.05). Conclusion Th1/Th2 cytokines participate in normal pregnance. Excess Th1 cytokines and activated immune cells result in Pre- eclampsia. 【Key words】Pre- eclampsia Th1 T