bcl2、bax、CD57在乳腺髓样癌中表达及其临床意义的研究 - 副本.pdfVIP

bcl-2,bax,CD57在乳腺髓样癌中表达及其临床意义的研究 摘要 目的:探讨细胞凋亡相关基因bcl-2,bax蛋白及CD57在乳腺典型髓样癌、 不典型髓样癌和硬癌中表达的临床意义。方法:通过TUNEL法检测三型乳腺癌中 凋亡细胞数量及分布比例的差异，并采用S-P法检测三型乳腺癌组织中bcl-2, bax蛋白及癌周浸润的淋巴细胞中CD57抗原表达阳性率的差异。结果:定量测 定三型乳腺癌细胞凋亡均数、面密度均数、面数密度均数及实际面数密度均数4 项指标，两型髓样癌显著高于硬癌 (P0.05);检测三型乳腺癌bcl-2,bax蛋白 及癌周浸润的淋巴细胞CD57抗原阳性率，bcl-2与CD57髓样癌显著高于硬癌 (P0.05)bax在三型乳腺癌中无显著差异 (P0.05).结论:髓样癌尤其是 典型髓样癌临床预后比硬癌好，与细胞凋亡水平高有关;髓样癌尤其是典型髓样 癌癌周浸润的淋巴细胞CD57抗原阳性率比硬癌高:凋亡相关基因bcl-2儿ax的 失衡及A-NK细胞的作用与乳腺髓样癌的预后有一定关系。 关键词:乳腺髓样癌 细胞凋亡 bcl-2蛋白 bax蛋白 CD57抗原 预后 Studiesofclinicalsignificanceofexpressionofbcl-2,baxandCD57 in the medullary breast carcinoma Abstract Objectives Toexploreclinicalsignificanceofexpressionof apoptosiscorrelationalgenes-bcl-2、baxproteinandCD57inbreast typicalmedullarcarcinoma，atypicalmedullarcarcinomaandscirrhous carcinoma.Methods Thespecimensfrom61casesofbreastcancerwere detectedbyTUNELandS-Pimmunohistochemicalmethod. Results Themean ofapoptosiscells、area-density、 area-numerical-densityandreal area-numerical-densityofmedullarycarcinomaweresignificantlyhigher thanthatofscirrhouscarcinoma (P0.05)，Theexpressionofbcl-2and CD57proteininmedullarycarcinomaweresignificantlyhigherthanthat ofscirrhouscarcinoma(PO.05).Theexpressionofbaxproteinshowsno differenceinthreekindsofbreastcarcinoma (P0.05).Conclusions Prognosisofmedullarycarcinomaespecialtypicalmedullarycarcinomais betterthanthatofscirrhouscancinoma,whichcorrelatewiththeirhigher apoptosislevel;TheexpressionofCD57proteininmedullarycarcinoma especialtypicalmedullarycarcinomawerehigherthanthatofscirrhous carcinoma;仰optosiscorrelationalgene- bcl-2/baxoffbalanceand factionofNK-cellmayplayimportantroleinprognosisofmedullary breastcarcinoma. 仁keywords]Medullarybreastcarcinomaapoptosis proteinbcl-2 proteinbax antigenCD57 prognosis 引