离子液体预处理影响纤维素微观结构和酶解行为的研讨.pdfVIP

离子液体预处理影响纤维素微观结构和酶解行为的研究 李秋瑾 殷友利 齐崴 苏荣欣 何志敏* （天津大学化工学院化学工程研究所 天津 300072 ） 摘要 以Whatman No.1滤纸为研究对象，设计了离子液体[AMIM]Cl预处理滤纸的实验方法以实现纤维素的高效 酶解糖化。在[AMIM]Cl中滤纸完全溶解，经水洗沉淀得到离子液体溶解后的再生纤维素，回收后的离子液体可 重复利用。预处理后底物酶解的可溶性糖转化率在24h时高达99.8% ；较之同样条件下未经预处理底物酶解糖转 化率（63% ）有飞跃性的提升。本文还考察了离子液体[AMIM]Cl预处理对纤维素结构及形态的影响，结果表明： [AMIM]Cl预处理后滤纸纤维素分子氢键减弱；结晶度明显下降，结晶型态由纤维素I型转变为纤维素II型；由规 整的平行排布转变为疏松有孔的无序形貌。正是离子液体预处理引起的纤维素微观与宏观结构性质的显著改变使 得再生后纤维素酶解的可溶性糖转化率大大提高。 关键词 纤维素；离子液体；预处理；酶解 Enzymatic Hydrolysis of Filter Paper with an Ionic Liquid Pretreatment Step * LI Qiujin ，YIN Youli ，QI Wei，SU Rongxin， HE Zhimin (Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072) Abstract Whatman No.1 filter paper was subjected to an ionic liquid (IL) [AMIM]Cl pretreatment step to obtain a high conversion of soluble sugars during enzymatic hydrolysis of cellulose. Whatman filter paper dissolved completely in [AMIM]Cl, followed by precipitating into deionized water to obtain the regenerated cellulose. The ionic liquid can be recycled for further use. The high performance liquid chromatography (HPLC) analysis results indicated that after 24h, the sugar released of IL-treated filter paper was 99.8%, much higher than that of the original substrate, which was only 63%. Structure, crystallinity and morphology of the IL-treated filter paper were also investigated as follows: the hydrogen bonding, detected by FTIR, was decreased; the crystallinity, monitored by XRD, changed significantly from crystalline