miRNA在喉癌中作用机制的研究进展.docVIP

miRNA在喉癌中作用机制的研究进展 彭永华杨文飞卢绍伟兰贤斌彭韶平 耳鼻咽喉头颈外科 关键词： 喉癌;微小RNA;生物行为;作用机制; 作者简介：彭韶平，E-mail:psp666@126.com 收稿日期：2017-04-18 Mechanism research progress of miRNA in laryngeal carcinoma PENG Yonghua YANG Wenfei LU Shaowei Abstract： MiRNA (miR) is a non-coding small RNA of 18to 24nuclcotidcs in length. By pairing with 3’ UTR of its target mRNA molecule, miRNA regulatcS the target mRNA molecule in the translation level or directly lead to its degradation. Each miRNA may target several to several hundred mRNAs, of which 30%of the mR-NA is regulated by miRNAs. Some studies have shown that miRNA expression disorders play an important role in the development and progression of various tumors and may be the ideal target for tumor therapy. It has been shown that abnormal expression of RNA (miRNA) plays an important role in the occurrence and development of laryngeal cancer. Wang Ping found 47kinds of miRNA expression abnormalities in laryngeal cancer tissue with microarray chip detection, that of which high expression were 23kinds and low expression were 24kinds. MiRXAs that arc abnormally expressed in laryngeal squamous cell carcinoma are expected to be a new marker for early diagnosis of laryngeal cancer. In this review, we summarized the progress in recent three years on the association of miRNA and the biological behaviors of laryngeal cancer. Keyword： laryngeal carcinoma; microRNA; pathogenesis; Received： 2017-04-18 miRNA (miR)是一类长18?24个核苷酸的非编码小RNA,通过与其靶mRNA分子 的3’ UTR互补配对，使目标mRNA分子受到翻译水平调控或直接导致其降解U1。 每个miRNA可能靶向数个到数百个mRNA,其中30%的mRNA受到miKNA的调控U1。 有学者研宄表明miRNA表达失调在各种肿瘤的发生、发展过程中起着重要的作用, 可能成为理想的肿瘤治疗靶点。目前的研究结果显示，喉癌组织中有多种miRNA 的表达异常，王苹等m用微阵列芯片技术检测喉癌组织，发现47种miRNA表达 异常其屮高表达和低表达分别为23种和24种。在喉癌组织屮异常表达的miR-NA 有望作为喉癌的早期诊断的新标志物，而某些miRNA更是有望成为治疗喉癌的 潜在靶点。 miR-21与喉癌 有实验结果表明，miR-21过表达与多种上皮系统来源恶性肿瘤的发生及其恶性 生物学表型有相关性，在乳腺癌、喉癌等许多实体瘤的组织或细胞中高表达位1。 范钰等通过下调miR-21的表达后培养人肿瘤Hep-2细胞，发现反义寡核苷酸 转染组Hep-2细胞增殖能力变慢、相关克隆能力降低，miR-21下调可对喉癌细 胞增殖、侵袭能力产生负面影响。细胞培养研究结果显示，miR-21的上调增强 喉癌细胞增殖能力，而它的下调会减少喉癌细胞数量Ul。BTG2是miR21的靶棊 因