1细胞Toll样受体2和MyD88以及TNF-α表达的影响.docVIP

1细胞Toll样受体2和MyD88以及TNF-α表达的影响 第26卷第l0期 2006年l0月 南京医科大学(自然科学版) ACTAUNIVERSITATISMEDICINALISNANJING(NaturalScience?921? 胸腺肽1对THP一1细胞Toll样受体2和MyD88以及TNF.仪表达的 影响 周苏明,周静,程蕴琳,吴文溪 (南京医科大学第一附属医院老年医学科,普外科,江苏南京210029) [摘要]目的:观察人THP—l细胞与胸腺肽Otl(Tct1)孵育后,予细菌脂蛋白(BLP)刺激后,Toll样受体2(TLR:),髓系分化因子 88(MyD88)蛋白的表达及细胞因子TNF—Ot的变化.方法:实验采用人THP—l细胞系进行细胞培养,分为4组:对照组为THP—l 细胞不加任何刺激:BLP组以l000ng/mlBLP刺激2h;Tdl组为THP.1细胞加l000ng/mlTcd孵育24h,不加BLP刺激; Tdl+BLP组为THP—l细胞加l000ng/mlTdl孵育24h后再加l000ng/mlBLP刺激2h.以Westernblot分析TLR,MyD88 蛋白含量.ELISA法测定TNF.Ot.结果:与对照组相比,BLP组和Tcd+BLP组的TNF.Ot均显着增加,而Tcd组无显着改变;在 TLR和MyD88的表达上.Tdl组和Tdl+BLP组均高于对照组和BLP组.结论:胸腺肽Otl可增加THP—l细胞TLR,MyD88蛋 白的表达.其对免疫细胞的影响与BLP诱发炎症反应的途径不完全一致. [关键词]胸腺肽dl;细菌脂蛋白;Toll样受体2;肿瘤坏死因子一Ot [中图分类号]R364.5[文献标识码]A[文章编号]1007-4368(2006)10-0921-04 EffectsofThymosin51ontheexpressionofToll—likereceptor2.MyD88andTNF-0cin THP-1celIIine ZHOUSu-ming,ZHOUJing,CHENGYun-lin,WUWen-xi (DepartmentofGeriatrics,DepartmentofGenerdSurgery,theFirstAffiliatedHospitalofNJMU,Nanjing 2』DD29,China) [Abstract]Objective:TostudytheeffectsofthymosinOt1ontheexpressionofToll-likereceptor2(TLR2),myeloiddifferentiation factor88(MyD881andTNF-ctinhumanTHP-lcelllinestimulatedbybacteriallipoprotein(BLP)withdifferentways.Methods:Human THP一1cellswereincubatedanddividedinto4groups:controlgroup(incubatedinRPMI一1640cellculturefluidwithnostimulus), BLPgroup(stimulatedwith1000ng/mlofBLPfor2h),Tcdgroup(incubatedwith1000ng/mlthymosindlfor24h),andTcd+ BLPgroupfstimulatedwith1000ng/mlofBLPfor2hafterincubatedwiththymosinOtl1.TLR2,MyD88proteinconcentrationwere detectedusingwesternblotanalysis.andTNF—OtwasdetectedusingavailableELISAkit.Results:TheconcentrationofTNF—Otin Tctlgrouphadnochangecomparedtocontrolgroup.ItincreasedinBLPgroupandTcd+BLPgroup.whiletherewasnodiffer- encebetweenbothgroups.TLR2andMyD88proteinexpressioninTcdgroupandTctl+BLPgroupwereup—regulated.Conclusion: Thymosin1up—regulatestheexpressionofTLR2andMyD88proteininTHP-1cellline.ThemechanismsforincreaseofTNF—etsyn— thesisinTHP一1cellscausedbyTctlmayb