关于大鼠中脑导水管周围灰质内gaba与阿片μ受体共存能神经元的观察.pdf

关于大鼠中脑导水管周围灰质内GABA 与阿片μ 受体共存能神经 元的观察 【关键词】GABA 关键词: GABA;受体，阿片样，μ ;水管周灰质;显微镜检查，共焦;免疫组 织化学;大鼠 摘 要:目的观察中脑导水管周围灰质（PAG）内是否存在γ -氨基丁酸 （GABA）与阿片μ 受体（MOR）共存神经元. 方法 免疫荧光组织化学双重染色 技术，染色结果在激光扫描共聚焦显微镜下观察. 结果 GABA 阳性神经元主要 见于PAG 中、尾段内侧区和腹外侧区，阳性神经元多散在分布并多为小型，中 型者较少;散在分布的MOR 阳性神经元主要见于PAG 中、尾段的腹外侧区，多为 中、小型神经元;散在并同时呈GABA 和MOR 双重染色阳性的中、小型神经元主 要见于PAG 中、尾段的腹外侧区. 结论 PAG 内有GABA 与MOR 共存的神经元， 提示内源性阿片物质对PAG 内的GABA 能神经元可能有调控作用. Keywords:GABA;receptors，opioid，mu;periaqueductal gray;microscopy，confocal;immunohis-tochemistry;rats Abstract:AIM To observe whetherγ -aminobutyric acid （GABA）andμ opioid receptor （MOR）co-exist in neurons of the periaqueductal gray （PAG）in the rat.METHODS Im-munofluorescent histochemical double- staining technique was used.The staining results were observed with laser scanning confocal microscope.RESULTS GABA-immunoreactive （GABA- IR）neurons were mainly seen in the medial and ven-trolateral subregions at the middle and caudal levels of the PAG.Most of the sparsely distributed GABA-IR neurons were small-sized and the number of medium-sized GABA-IR neurons was small.Scattered MOR-IR neurons were mainly observed in the ventrolateral subregion at the middle and cau-dal levels of the PAG.Most of the MOR-IR neurons were small-and medium-sized.Small-and medium-sized neurons simultaneously showing both GABA-IR and MOR-IR were scattered in the ventrolateral subregion at the middle and cau-dal levels of the PAG.CONCLUSION There are GABA and MOR co-existing neurons in the PAG.The present results suggest that endogenous opioid-like substances might regu-late GABAergic neurons in the PAG. 0 引言 以往的研究证明，中脑导水管周围灰质（PAG）内5-羟色胺（5-HT）能下 行投射系统在调控伤害性信息向中枢的传入过程中发挥着重要作用，而γ -氨基 丁酸（GABA）对5-HT 能神经元具有抑制性调控作用［1-4］ .有人曾报道激活 中枢神经系统内的阿片μ 受体（μ opioid receptor，MOR）具有直接调节GABA 能神经元活动的作用［5］ .激活MOR 后调节GABA 能神经元活动的结果能使 PAG 的下行投射神经元摆脱GABA 能神经元的抑制（disinhibition，脱抑制） 并发挥镇痛作用［6］ .但PAG 内的GABA 能抑制性神经元是否呈MOR 阳性