利用大粒突变体进行小麦籽粒大小和产量性状的qtl研究-qtl study on grain size and yield traits of wheat using large-grain mutants.docxVIP

利用大粒突变体进行小麦籽粒大小和产量性状的 QTL 分析markers on chromosome 2D to detect polymorphisms in the F2 generation. After screening the two F2 populations using the polymorphic markers, linkage maps of 2D were constructed with a total genetic distance of 115.2 cM for MY and 250.4 cM for MS. The map derived from the MY population was generally in keeping with that from MS population. 6 polymophic markers on 7B were were used for map construction of 7B.Seven QTLs for grain size traits were identified in F2:3 of MY on chromosome 2D and 7B, while 4 QTLs for grain size traits were identified in F2 and F2:3 of MS on chromosome 2D.QTLs for grain size (QGw、QGlw、QFfd and QTgw) in MY and MS formed one QTLcluster in each population on chromosome 2D, which in MY covered the marker wmc41 and in MS was near wmc41. There was also a QTL cluster for yield trait (PH, SL and SN) detected in MS on 2D. A QTL for plant height in the F2:3 of MY was detected between Xwmc76 and Xwmc426 on chromosome 7B, with a contribution of 9.33%. There was a QTL cluster for grain size trait (TGW, GW and FFD) on 7B.Surprisingly, the addictive effects of QTLs for GW and TGW were negative, indicating that the effects of the QTLs were decreased in M8008, though the phenotypes of GW and TGW of M8008 were higher than of either parent (YN15 or SJZ54). In other words, the QTLs had increased effects in smaller size cultivars YN15 and SJZ54.Key words: Common wheat; Mutant; Simple sequence repeat (SSR); Quantitative trait locus (QTL); Grain size trait; Yield trait4符号说明英文缩写Abbr.英文名称English中文名称ChineseAFLPAmplification Fragment LengthPolymorphism扩增片段长度多态技术APAmmonium Persurface过硫酸铵BisN, N-methylene-bis-acrylamide甲叉双丙烯酰胺bpbase pair碱基对CSSLChromosomal segment substitution line染色体片段代换系ddH2Odistilled and deionized water重蒸水DNAdeoxyribose nucleic acid脱氧核糖核酸dNTPdeoxynucleoside triphosphate脱氧核苷三磷酸EBEthidium bromide溴化乙锭EDTAethylenediaminetetra acetic acid乙二胺四乙酸EMSethyl methane sulfonate甲基磺酸乙酯ESTexpressed sequence tag表达序列标签FSSfertile spikelet