2-甲氧基雌二醇对全脑缺血大鼠HIF-1α及RTP801作用研究.docVIP

2-甲氧基雌二醇对全脑缺血大鼠HIF-1α及RTP801作用研究 　　[摘要] 目的 探讨2-甲氧基雌二醇（2ME2）对全脑缺血大鼠HIF-1α及凋亡相关基因RTP801的作用。 方法 将成年雄性SD大鼠104只随机分为对照组（n=8）、全脑缺血组（n=48）和全脑缺血2-甲氧基雌二醇干预组（n=48），后两组按再灌注时间不同各分为6个亚组。采用改良的Pulsineli 4-VO方法行全脑缺血大鼠模型制作。Nissl染色进行海马神经元计数，免疫组化及RT-PCR技术进行HIF-1α及RTP801 mRNA表达检测。 结果 全脑缺血2ME2干预组与全脑缺血组相应时间点亚组相比，神经细胞计数明显增加，HIF-1α表达减少，RTP801 mRNA表达减低（P＜0.05）。 结论 在全脑缺血急性期，2ME2抑制HIF-1α及凋亡相关基因RTP801表达，具有一定的神经保护作用。 　　[关键词] RTP801；全脑缺血；缺氧诱导因子-1；2-甲氧基雌二醇 　　[中图分类号] R743.31 [文献标识码] B [文章编号] 1673-9701（2012）01-006-03 　　 　　Effects of 2ME2 on hypoxia-inducible factor 1α and apoptosis-related gene RTP801 in hippocampus following the global cerebral ischemia in rats 　　PENG Jinghua1 ZHANG Hongri2 HUANG Lina1 LI Yun3 　　1.Department of Neurology, the First Affiliated Hospital to Henan University of Science and Technology, Luoyang 471003, China;2.Department of Neurosurgery, the First Affiliated Hospital to Henan University of Science and Technology, Luoyang 471003, China; 3.Department of Emergency, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China 　　 　　[Abstract] Objective To investigate the effect of 2ME2 on the hypoxia-inducible factor 1α and apoptosis-related gene RTP801 in hippocampus following the global cerebral ischemia in rats. Methods One hundred and four adult male Sprague-Dawley rats were randomly divided into 3 groups: sham group (n=8), global cerebral ischemia group (n=48) and global cerebral ischemia + 2ME2 treatment group (n=48), and the rats of GI group and GI + 2ME2 group were sacrificed after perfusion. The 4 vessel occlusion (4-VO)global ischemia rat model was produced. Nissl staining was used to perform quantitative analysis of cell numbers. Immunohistochemistry staining and RT-PCR provided the information of the distribution of positive cells to HIF-1α protein and the expression of RTP801 mRNA. Results The number of cells from GI + 2ME2 group was significantly higher than that from GI group (P???0.05). 2ME2 significantly suppressed the levels of HIF