IL-6、TNF-α测定与COPD患者不同营养状态相关性研究.docVIP

IL-6、TNF-α测定与COPD患者不同营养状态相关性研究 　　【摘要】 目的 研究白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）对慢性阻塞性肺疾病（COPD）患者营养状态的影响。方法 选择COPD临床稳定期患者49例，根据体质量指数（BMI）、理想体质量百分比（NW%）、肱三头肌皮褶厚度（TSF）、上臂中部臂围（MAC）、血清白蛋白（ALB）及总淋巴细胞（LYM）等营养参数，分为COPD营养不良组（COPDⅠ组）25例，COPD非营养不良组（COPDⅡ组）24例，正常对照组25例。用酶联免疫吸附实验法（ELISA）测定各组血清IL-6、TNF-α，并分析血清IL-6、TNF-α与各营养参数之间的相关性。结果 COPD组BMI、NW%、MAC、TSF低于对照组，IL-6、TNF-α高于对照组，差异有统计学意义（?P? 　　【关键词】 慢性阻塞性肺疾病； 营养不良； 白细胞介素-6； 肿瘤坏死因子-α?? 　　Clinical investigation of relation between the serum levels of IL-6 and TNF-α in chronic obstructive pulmonary disease patients ?MENG Hong-qiong, WANG Ru, ZHANG Yao-Yun,et al. Kunming Yan?an hospital, Yunnan 650051, China?? 　　【Abstract】 Objective To investigate the correlation between the serum interleukin-6(IL-6) levels and tumor necrosis factoe-alpha(TNF-α) on nutrition state in the patients with chronic obstractive pulmorary disease(COPD).Methods Fourty-nine COPD patients in stable stage and twenty-five healthy controls were studied. Nutritonal parameters, including body mass indox(BMI), percentage of normal body mass(NW%), tricepsskin-fold thickness(TSF), mid-upper arm circumference(MAC), serum albumin(ACB), told lymphocyte couts(LYM) were determined. 25 COPD patients were divided into malnutrition group(groupⅠ), 24 COPD patients non-malnoutrtion group (group Ⅱ). Serum IL-6 and ?TNF-?α were measured by enzyme-linked immunonsorbent assay(ELISA) at the same time. The correlation between IL-6 and nutritional parameters as well as TNF-αwas analyzed.Results BMI, NW%, TSF and MAC in COPD patients were significantly lower than those in healthy controls, while the levels of IL-6 and TNF-αin cases were significantly higher than in controls(?P? 　　1.2 研究方法? 　　1.2.1 反映营养状况的人体测量学指标（身高、体重、TSF、MAC）参照文献方法进行测量，NW%对照理想体重表计算。? 　　1.2.2 实验器材及试剂 所有受试者前1天8 pm开始进食，次日起7 am空腹抽取肘正中静脉血5 ml，标本在1 h内以2000 r/min离心10 min，分离血清标本于-20 ℃冻存待测。ALB用自动生化仪测定，LYM用血细胞计数仪测定，血清IL-6、TNF-α的测定采用酶联免疫吸附法（ELISA），试剂盒为美国GenZyme公司产品（96孔）批号（041128 050416 200611102）。? 　　1.3 统计学处理 采用SPSS 1