运动对大鼠心肌FOS和HSP70表达影响.docVIP

运动对大鼠心肌FOS和HSP70表达影响 　　摘 要: 目的:探讨不同运动方式对心肌FOS蛋白和HSP70表达的影响,从而为运动增进心脏健 康提供理论依据。方法:在不同运动方式动物模型的基础上,采用免疫组化染色方法检测FO S蛋白和HSP70的表达。结果:一次急性运动中,运动后1 hFOS蛋白明显增加,运动后3 h达 到峰值,运动后5 h以后恢复到安静时水平。 HSP70运动后1 h、3 h均明显增加,运动后5 h 达到高峰,持续到运动后12 h;长期耐力运动后,FOS蛋白运动后即刻表达显著增加,运动 后24 h表达有所减少,HSP70运动后即刻和运动后24 h均显著增加。结论:急性运动应激后 ,HSP70表达高峰晚于FOS蛋白,表达时限稍长,FOS蛋白的表达诱导了HSP70的表达。长期耐 力运动应激引起FOS蛋白大量表达,是心肌肥大的一个始动因素;长期耐力运动诱导HSP70高 表达,有助于减少心肌的损伤、促进损伤后的恢复。 　　关键词:急性运动;耐力运动;FOS;HSP70 　　中图分类号:G804.7 　　文献标识码:A 　　文章编号 :1007-3612(2010)02-0064-04 　　 　　Effect of Exercise on the Expression of Cardiac FOS and Heat Sho ck Protein 70 in Rats 　　ZHANG Li?qin?1,WANG Yun?hong?2,ZHAO Ming?hua?2,XI Li?li?2 　　(1.Inner Mongolia Institute of Sports Science, Hohhot 010030 , Inner Mongolia China;?2.Capital Institute of Physical Education, Bei jing 100088 China) 　　Abstract: The objective of the thesis is to discuss the effect of different mode s of exercise on the expression of cardiac FOS protein and HSP70. Thus it provid es the theory basis for the exercise promoting the cardiac health. It adopts Imm unohistochemistry method to examine FOS protein and HSP70 based on the different 　　animal exercise models. It finds that in acute exercise, FOS protein increases 　　obviously in one hour after exercise. FOS protein achieves the peak in three ho urs after exercise, and restores to the level of control group in 5 hours after 　　exercise. HSP70 significantly increases in one hour after exercise and three ho urs after exercise. HSP70 achieves the peak in five hours after exercise, and c ontinues to 12 hours after exercise; after long?term endurance training, FOS 　　protein is remarkably increased in 0 hour after exercise. FOS protein is reduced 　　in 24 hours after exercise. HSP70 obviously increases in 0 hour after exercise 　　group and 24 hours after exercise group. In conclusion, after acute exercise st ress,the expression peak of HSP70 is later than that of FOS protein, and the 　　expression time is slightly longer. FOS