比索洛尔对动脉粥样硬化大鼠血no及血管mmp9 vegf表达的影响-effects of bisoprolol on the expression of no and vascular m mp9 vegf in atherosclerotic rats.docxVIP

P0.05,C组与B组无明显统计学差异，P0.05，D组和E组与B组相比，P值均小于0.05，D组和E组相比较有统计学意义P0.05。5.各组血管MMP9的平均光密度值：A组：11.936±5.045，B组：137.577±32.548，C组：123.562±27.071，D组：83.691±20.233，E组：46.510±18.087。B组与A组相比P0.05,C组与B组无明显统计学差异，P0.05，D组和E组与B组相比，P值均小于0.05，D组和E组相比较有统计学意义P0.05。结论：1.通过给予大鼠高脂饲料及定时定量给予腹腔注射VitD可成功复制大鼠动脉粥样硬化模型。2.本实验中可见大鼠血NO及NO合酶在动脉粥样硬化模型组中明显降低，表明内皮功能障碍参与了动脉粥样硬化发生发展。3.MMP9在动脉粥样硬化模型大鼠主动脉组织中明显升高，表明细胞外基质降解与动脉粥样硬化及斑块不稳定有密切关系。4.VEGF在动脉粥样硬化模型大鼠主动脉组织中明显升高，提示动脉粥样硬化的形成与血管内皮细胞过度增生有关。5.比索洛尔干预后，血NO及NO合酶明显升高，组织内VEGF及MMP9明显下降，表明比索洛尔能改善粥样硬化血管的血管内皮功能，防治内皮增生，减少细胞外基质的降解，进而发挥抗AS的作用。关键词：比索洛尔，动脉粥样硬化、血管内皮生长因子，基质金属蛋白9，NO，NOSEffectsofbisoprololonbloodNOandtheexpressionofvascularMMP9,VEGFinatherosclerosisratsAbstract：Objective：ThemainpurposeofthisexperimentistoobservetheeffectsofdifferentdosagesofbisoprololonbloodNOandvascularMMP9,VEGFexpressioninatherosclerosisrats.Methods:1.fiftysix-weeks-oldwisterrats(weight180±40g)distributeintofivegroupsrandonmly:(1)GroupA(Blankcontrol):tobreedwithecumenicfeed;(2)GroupB(Atherosclerosisgroup):tobreedwithsuperlipoidfeed(include80.3%ecumenicfeed,2%cholesterol,0.5%sodiumcholate,0.2%propylthiouracil,10%lard,7%whitesugar),andtoinjectvitaminDwhichvolumedoseis600thousandu/kginthreedaysinperitonealcavityatleastevery30days.(3)GroupC(Bisoprololinterventiongroup1):tobreedwithsuperlipoidfeed,andtoinjectvitaminDwhichvolumedoseis600thousandu/kginthreedaysinperitonealcavityatleastevery30days,andtointragastricadministrationwithbisoprololwhichdoseis1.25mg/kg/dperonerat.(4)GroupD(Bisoprololinterventiongroup2):tobreedwithsuperlipoidfeed,andtoinjectvitaminDwhichvolumedoseis600thousandu/kginthreedaysinperitonealcavityatleastevery30days,andtointragastricadministrationwithbisoprololwhichdoseis2.5mg/kg/dperonerat.(5)GroupE(Bisoprololinterventiongroup3):tobreedwithsuperlipoidfeed,andtoinjectvitaminDwhichvolumedoseis600thousandu/kginthreedaysinperitonealcavityatleastevery30days,andtointragastricadministrationwithbisoprololwhichdoseis5.0mg/kg/dperonerat.2.After13weeks,4mlbloodwasextractedfromchamberso