雌核发育红鲫遗传概貌及生化遗传标记的分析-genetic profile of gynogenetic red crucian carp and analysis of biochemical genetic markers.docxVIP

PAGE PAGE 10Study on Genetic Profile and Biochemistry Genetic Markers of the Homozygous carassius auratus red variety ABSTRACTObjectives Carrassius aurratus red variety as laboratoryanimal has considerable application values in acute toxicology, heavy metal contamination of aqua circumstance and pollution monitor of pesticide. Clones of homozygous carassius auratus red variety are produced from artificial gynogenesis in our laboratary. Isozyme,blood biochemistry and karyotype of the homozygous carassius auratus red variety are analyzed for its national register as laboratory animal and providing detection standard for itself,and part of genetic profile and biochemistry genetic markers set up.Part 1 Analyses of gel electrophoresis of isozymes methods MDH、LDH、EST and SOD isozyme of the red cell of homozygous carassius auratus red variety and the wild ones are analyzed by polyacrylamide gradient gel vertical electrophoresis.Results MDH isozyme electrophoretic bands of six homozygous carassius auratus red variety and four wild ones’ are three,and the band pattern are the same. MDH isozyme electrophoretic bands of two wild ones are five.LDH isozyme electrophoretic bands of homozygous carassius auratus red variety and wild ones’ are nine,and the band pattern are the same. EST isozyme electrophoretic bands of homozygous carassius auratus red variety are two or three and wild ones’ are from one to three. SOD isozyme electrophoretic bands of homozygous carassius auratus red variety are eight and wild ones’ are six,and the former have two morebands(SOD-3、SOD-8) than the latter,but the respective of the twofishes are the same.Conclusion SOD-3 and SOD-8 of the SOD isozyme electrophoretic bands can be as the biochemistry genetic markers of homozygous carassius auratus red variety.MDH,LDH and SOD isozyme electrophoretic bands of homozygous carassius auratus red variety are respectively three,nine and eight.Part 2 Biochemistry analyses of the blood Methods Blood glucose, cholest