白芍配方颗粒指纹图谱及7种主要成分含测定研究.docVIP

白芍配方颗粒指纹图谱及7种主要成分含量测定研究 　　【摘要】 目的：建立白芍配方颗粒的指纹图谱，并对其7种主要成分进行含量测定，为白芍配方颗粒的质量控制提供实验依据。方法：本研究采用HPLC-DAD法，色谱柱为Inetsil-ODS-SP C18（4.6 mm×250 mm，5 μm），流动相为乙腈（A）?C0.1%甲酸水溶液（B）进行梯度洗脱，流速0.8 ml/min，柱温30 ℃，检测波长233 nm和270 nm，测定了10批次白芍配方颗粒。结果：指纹图谱检测结果，共确定白芍配方颗粒20个共有峰，各批次白芍配方颗粒指纹图谱与对照图谱相比，相似度均在0.900以上；含量测定结果表明，7种主要成分的峰面积和质量浓度的线性关系良好，r均大于0.999，平均加样回收率96.0%～98.6%。?Y论：本文建立了白芍配方颗粒的HPLC指纹图谱和和其中7种成分含量测定方法，可以为白芍配方颗粒质量控制提供参考。 中国论文网 /6/viewhtm　　【关键词】 白芍配方颗粒； HPLC； 指纹图谱； 质量控制 　　doi：10.14033/j.cnki.cfmr.2017.36.001 文献标识码 A 文章编号 1674-6805（2017）36-0001-04 　　【Abstract】 Objective：A method of fingerprint and simultaneous quantitative analysis of seven components by high performance liquid chromatography（HPLC） was developed and validated for quality evaluation for Baishao Granules.Method：The method was performed by HPLC using Inetsil-ODS-SP C18（4.6 mm×250 mm，5 μm） with a mobile phase of consisted of acetonitrile（A）-0.1% formic acid（B） in gradient mode at a flow rate of 0.8 ml/min；column temperature 30 ℃；wavelength set at 233 nm and 270 nm.10 batches of Baishao Granules were measured.Result：For fingerprint analysis，20 peaks were selected as the common peaks to evaluate the similarities among 10 batches of Baishao Granules collected from different regions with similarity higher than 0.900.Additionally，simultaneous quantification of seven markers，including gallic acid，methyl gallate，catechin，albiflorin，paeoniflorin，pentagalloylglucose and benzoylpaeoniflorin，were performed and the obtained data demonstrated that the method had achieved desired linearity（r0.999），average recovery were ranged from 96.0% to 98.6%.Conclusion：The results indicated that HPLC fingerprint and simultaneous quantitative analysis of seven components was applicable in quality control of Baishao Granules. 　　【Key words】 Baishao Granules； HPLC； Fingerprint analysis； Quality control 　　First-author’s address：The Second Hospital of Fujian Medical University，Quanzhou 362000，China 　　中药配方颗粒是中药材临床应用的另外一种形式，是符合炮制要求的中药饮片在中医药理论指导下，