双歧杆菌对新生鼠坏死性小肠结肠炎回肠形态学及inos egf基因表达影响-effect of bifidobacterium on ileum morphology and inos egf gene expression in neonatal rats with necrotizing enterocolitis.docxVIP

beforeandaftermodeled;themortalityofthethreegroupsduringmodeld;ileumtissueswereaffixedbyformaldehydesolution,imbeddedinparaffin,andstainedwithH.E.ThetissuesofeachgroupwerescoredaccordingtotheirinjuryandNECincidenceswereassessed;TotalRNAwereextractedfromtheileumtissueswhichwereconservatedin-80℃,andreversetranscriptedintocDNA,thegeneexpressiondifferenceofiNOS、EGFbetweeneachgroupweretestedbyfluorescentquantitationPCR.6.statisticsmethod:ThedataswerecalculatedbySPSS13.0.measurementdataswerepresentedbymean±standarddeviation（?x±s）,t-testorWilcoxonranksumtestwasusedforgroupcomparison,χ2-testwasusedforratecomparison,itwasconsideredsignificantdifferencewithP0.05.Results:Aftermodeled,theweightofgroupBratswasloweraftermodeled［-（0.23±0.59）g］,whilethegroupAratswerelightlyhigher［（0.41±0.59）g］,andtheweightincrementingroupCratswerenormalduringtheexperimentaltime（2.45±0.34）g］.ThemortalityofgroupA,BandCwas7.69%、15.38%、0,respectively,P0.05ingroupcomparison,differencewasnotsignificant.TheNECincidenceofgroupA,BandCwas38.46%（5/13）、84.62%（11/13）和0（0/13）,P0.05ingroupcomparison,differencewassignificant;Theilieumpathologyinjuryscore（?x±s）ofgroupA,BandCwas1.50±0.52、2.35±0.41、0.27±0.37,respectively;Ofthetotal,thescoreofgroupAdecreasedobviouslythangroupB,butwerehigherthangroupC,P0.05.TheresultofgeneexpressiontestedbyfluorescentquantitationPCRshowedasfollows:ForiNOS:iNOSofgroupAandBwereheightenedsignificantcomparedwithcontrolgroupC,thevalueofAgroupwas2.74timesgreaterthangroupC,andthevalueofgroupBwas12.46timesasgreatasgroupC,P0.05;ThevalueofgroupBwaspromotedsignificantlywhencomparedwithgroupA;ForEGF:BoththegroupAandBdecreasedthangroupC,groupAwas0.52timesgreaterthangroupC，P0.05,butgroupBwas0.85timesasgreatasgroupC,P0.05;TherewasnosignificantdifferencebetweengroupAandB（P0.05）.Conclusions:TheearlypostnatalneonatescouldmimicexperimentalNECanimalmodelanalogousashumanthroughfeddingwithaspecialrodentformula,hypoxia(100%N2),4℃coldstimulusandfeddingLPSoflowconcentration.Thewayaddingbifidobacteriacoulddecre