mTOR信号通路在心肌肥大中作用.docVIP

mTOR信号通路在心肌肥大中作用 　　[摘要] 目的 检测雷帕霉素靶蛋白（mTOR）信号通路蛋白在压力超负荷性心肌肥厚及H9c2心肌细胞肥大时的表达，探讨mTOR信号通路对心肌肥大的影响。 方法 将16只雄性SD大鼠随机分为腹主动脉缩窄术（AAC）组和假手术组，每组各8只。AAC后2周，实施超声心动图检查及病理学检查。体外研究采用去甲肾上腺素（NE）诱导H9c2心肌细胞肥大。Western blot检测mTOR信号通路相关蛋白表达。 结果 与假手术组比较，AAC组大鼠左室舒张末期后壁厚度（LVPWd）、室间隔舒张末期厚度（IVSd）和心脏重量指数（HWI）明显升高，差异均有统计学意义（P 0.05）。Western blot检测发现，AAC组P-mTOR/mTOR蛋白表达比值明显高于假手术组，差异有统计学意义（P 0.05）。体外研究中，NE组P-mTOR/mTOR蛋白表达比值明显升高，与对照组比较，差异有统计学意义（P 0.05）。 结论 压力超负荷性心肌肥厚及H9c2细胞肥大时，mTOR信号通路相关蛋白表达增加，这提示mTOR信号通路在心肌肥大的发病过程中发挥重要作用。 　　[关键词] 雷帕霉素靶蛋白；信号通路；心肌肥大；腹主动脉缩窄术 　　[中图分类号] R541 [文献标识码] A [文章编号] 1673-7210（2018）03（a）-0016-04 　　[Abstract] Objective To detect protein the expression of mammalian target of rapamycin （mTOR） signaling in pressure-overloaded cardiac hypertrophy and H9c2 cardiomyocyte hypertrophy， and investigate effect of mTOR signaling on cardiac hypertrophy. Methods Sixteen SD rats were randomly grouped as the abdominal aorta coarctation （AAC） group and the sham group， with 8 rats for each group. Two weeks after AAC， echocardiography and histological analysis were performed. Norepinephrine （NE） was used to induce H9c2 cardiomyocyte hypertrophy in vitro. Relative protein expressions were detected for mTOR signaling by western blot. Results Compared with the sham group， rats in the ACC group showed a significant increase in left ventricular posterior wall end-diastolic thickness （LVPWd）， interventricular septal end-diastolic thickness （IVSd） and heart weight index （HWI） （all P 0.05）. Western blot showed that there was a significant increased ratio of P-mTOR and mTOR protein expression in the AAC group compared with the sham group （P 0.05）. NE treatment significantly increased the ratio of P-mTOR and mTOR protein expression in vitro， with a significant difference compared with the control group （P 0.05）. Conclusion Protein expressions in mTOR signaling can be significantly increased in pressure-overloaded cardiac hypertrophy and H9c2 cardiomyocyte hypertrophy. mTOR signaling may play a ke