HPLC测定藏药巴尔达尔黄酮类成分含量及指纹图谱研究.docVIP

HPLC测定藏药巴尔达尔黄酮类成分含量及指纹图谱研究 　　[摘要]研究藏药巴尔达尔Hippophea gyantsensis黄酮类3种指标性成分进行含量测定，同时建立藏药巴尔达尔的HPLC指纹图谱，全面评价其内在质量。采用C18色谱柱（4.6 mm×250 mm，5 μm），以流动相乙腈-0.2%磷酸溶液梯度洗脱，检测波长360 nm，流速1.0 mL?min-1，柱温30 ℃，进样量10 μL；采用中药色谱指纹图谱相似度评价系统（2004A）软件进行指纹图谱分析。建立了10个批次藏药巴尔达尔的共有图谱，提取了12个色谱峰作为指纹图谱共有峰，成功测定藏药巴尔达尔中山柰素-3-O-β-D-槐二糖-7-O-α-L-鼠李糖苷、异鼠李素-3-O-β-D-槐二糖-7-O-α-L-鼠李糖苷、异鼠李素-3-O-β-D-葡萄糖-7-O-α-L-鼠李糖苷的含量。该研究首次采用HPLC建立藏药巴尔达尔的指纹图谱和多成分含量测定，该方法具有良好的精密度、稳定性和重复性，为江孜沙棘质量控制和评价提供依据。 　　[关键词]藏药；巴尔达尔；高效液相色谱法；指纹图谱；含量测定 　　[Abstract]This study is to establish an HPLC fingerprint and quantitative analysis of 3 components of Gyantse Seabuckthorn from different producing areas.The separation was developed on Shimadzu InertSustain C18column （4.6 mm×250 mm，5 μm） by gradient elution with acetonitrile and 0.2% phosphoric acid water as mobile phase at a flow rate of 1.0 mL?min-1；the detection wavelength was set at 360 nm and column temperature was set at 30 ℃. The data calculation was performed with similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine（Version 2004A）.The fingerprints of 10 batches of Gyantse Seabuckthorn were carried out by similarity comparison， and 12 chromatographic peaks were extracted as the common peaks of fingerprint，of which three main active ingredients were successfully determined.This is the first established fingerprint and multi-component quantitative determination of Gyantse Seabuckthorn by using HPLC.This method has good precision stability and repeatability that could provide basis for quality control and evaluation of Gyantse Seabuckthorn. 　　[Key words]Tibetan medicine； Gyantse Seabuckthorn； HPLC； fingerprint； determination 　　藏药巴尔达尔系胡颓子科植物江孜沙棘Hippophea gyantsensis（Rousi）Lian的干燥成熟果实，小乔木，主要分布在西藏中部一江两河流域[1]。《晶珠本草》记载沙棘应分为大、中、小3种 。“中者”称为巴尔达尔，果大如青稞，硬而干，金黄色，微油润，益血，与江孜沙棘相符[2]。四川省藏药材标准（2014年版）把江孜沙棘归为大沙棘[3]。其主要化学成分是黄酮类化合物、维生素C等，其中山柰素-3-O-β-D-槐二糖-7-O-α-L-鼠李糖苷，槲皮素-3-O-β-D-槐二糖-7-O-α-L-鼠李糖苷、槲皮素、山柰素、异鼠李素为其主要的黄酮类化合物[4-6]。其黄酮类化合物对血液系统、心血管系统、消化系统、免疫系统以及呼吸系统疾病