不同配伍比例芍药甘草汤成分肠吸收研究.docVIP

不同配伍比例芍药甘草汤成分的肠吸收研究 　　[摘要] 为探讨芍药甘草汤配伍机制与适宜的配伍比例，采用大鼠外翻肠囊模型、在体单向肠灌流模型，从肠吸收角度研究芍药甘草汤SG11（白芍-炙甘草1∶1）、SG31（白芍-炙甘草3∶1）、白芍水煎液S及炙甘草水煎液（G）中主要指标成分在大鼠离体、在体肠吸收情况，并进行比较。以HPLC测定上述供试品以及肠囊吸收样品、肠灌流样品中芍药苷（paeoniflorin，PF）、甘草苷（liquiritin，LQ）、甘草酸单铵盐（glycyrrhizin single ammonium salt，GL）的浓度，并计算肠吸收量及吸收参数。发现在外翻肠囊模型中，3种成分在十二指肠、空肠和回肠均有吸收，最佳吸收部位均为空肠，且SG11中相应成分的吸收率均显著高于单煎（Pa、表观吸收系数Paapp和吸收率均显著高于各单煎液，SG11中PF，GL 的各参数均显著高于SG31，LQ，各参数无差异。说明白芍炙甘草配伍有助于PF，LQ和GL的肠吸收，SG11较SG31各指标成分的吸收好。 　　[关键词] 芍药甘草汤；单向在体肠灌流；外翻肠囊模型；配伍 　　[Abstract] To study the compatible mechanisms and compatible proportion of Shaoyao Gancao decoction， the intestinal absorption of main ingredients in Shaoyao Gancao decoction SG11 （Baishao-Zhigancao 1∶1）， SG31 （Baishao-Zhigancao 3∶1）， Baishao water decoction S and Zhigancao （G） were investigated and compared using in vitro everted intestinal sac model and in situ single pass intestinal perfusion （SPIP） model. The concentration of paeoniflorin （PF）， liquiritin （LQ） and mono-ammonium glycyrrhizinate（GL） in test samples and samples of intestinal sac and intestinal perfusion was determined by HPLC. The intestinal absorptive amount and absorption parameters were calculated. Results showed that in the everted intestinal sac model， three ingredients could be absorbed by duodenum， jejunum and ileum， and the absorption in the jejunum was best for all 3 ingredients. The absorption rate of three ingredients in SG11 was significantly higher than that in single decoction（Pa， the apparent absorption coefficient Paapp and the absorption rate of three ingredients in SG11 were significantly higher than those in single decoction. Parameters of PF and GL in SG11 were significantly higher than those in SG31， but had no differences of LQ. It proved that the compatibility of Baishao and Zhigancao could improve the intestinal absorption of PF， LQ and GL. The absorption of each ingredient in SG11 was better than that in SG31. 　　[Key words] Shaoyao Gancao decoction； single pass intestinal perfusion； ever