不同PGPR菌株对松针叶绿素含量影响.docVIP

不同PGPR菌株对松针叶绿素含量的影响 　　摘要：笔者将荧光假单胞菌（Pseudomonas fluorescens）Q2-87、1M1-96、Pf-5和洋葱伯克霍尔德氏菌（Burkholderia cepacia）C23等四个PGPR菌株分别于2014年4月和5月接种于火炬松和湿地松苗根部;并于当年8月份利用手持式叶绿素测定仪测定两种松苗针叶的SPAD值。结果如下：①接种菌株Q2-87和1M1-96的火炬松苗针叶SPAD值显著高于清水对照;接种菌株Pf-5与接种菌株Q2-87和1M1-96的火炬松苗针叶SPAD值无显著性差异;②接种菌株Q2-87和1M1-96的湿地松苗针叶SPAD值显著高于清水对照。根据本文的实验结果和参阅文献，我们推断菌株Q2-87、1M1-96和Pf-5的代谢产物2，4-DAPG诱导火炬松和湿地松苗产生了系统诱导抗病性。 　　关键词：PGPR;湿地松;火炬松;叶绿素含量 　　中图分类号：Q939.96 文献标识码：A 文章编号：1004-3020（2015）04- 　　Effects of Different PGPR Strains on the Chlorophyll Content of 　　Pine Needle Leaf 　　Cai Sanshan（1） Chen Jingyuan（1） Hu Chenghui（1） Yang Jinhua（2） Fu Yun（3） Liu Gang（4） Hubei Academy of Forestry Wuhan 430075; 2. General Station of Hubei Provincial Forest Pests and Diseases Control and Quarantine Wuhan 430079; 3. Dangyang Municipal Forestry Bureau Dangyang 444100; 4. Songzi Municipal Forestry Bureau Songzi 434200） 　　Abstract： Four PGPR strains including Pseudomonas fluorescens Q2-87， 1M1-96， Pf-5 and Burkholderia cepacia C23， were inoculated on loblolly pine and slash pine in April and May， 2014，respectively; The SPAD values of pine seedlings were measured using SPAD 502 PLUS in August， 2014. The results were as follows： ①The SPAD values of the needle leaves of loblolly pine seedling inoculated with Q2-87 and 1M1-96 were significantly higher than that of loblolly pine seedling inoculated with water; That of loblolly pine seedling inoculated with Pf-5 had no significant difference with that of loblolly pine seedling inoculated with Q2-87 and 1M1-96; ②The SPAD values of the needle leaves of slash pine seedling inoculated with Q2-87 and 1M1-96 were significantly higher than that of slash pine seedling inoculated with water. According to the experimental results and the references， we estimate that ISR was induced by 2，4-DAPG in the two kinds of pine inoculated with Q2-87， 1M1-96 and Pf-5. 　　Key words： PGPR; Slash pine; Loblolly pine; Chlorophyll content 　　植物根际促生菌（简称PGPR）是指能够促进植物生长、防治病害、增加作物产量的微生物，