中介素153对急性肺损伤大鼠SPA及IL18影响.docVIP

中介素1-53对急性肺损伤大鼠SP-A及IL-18的影响 　　[摘要] 目的 探讨脂多糖致急性肺损伤大鼠肺泡灌洗液（BALF）中表面蛋A（pulmonary surfactant associated protein A，SP-A）浓度及肺组织单核巨噬细胞中白介素18（interleukin 18，IL-18）的表达水平及中介素1-53（intermedin1-53，IMD1-53）的影响。 方法 将36只雄性Vistar大鼠随机分为正常组（A组）、急性肺损伤组（B组）、中介素1-53干预组（C组），于2 h，4 h分别测定血气分析，后处死大鼠，测定肺组织湿/干比（W/D）、ELISA法检测BALF中SP-A的浓度、免疫组化法检测肺单核巨噬细胞内IL-18表达及各时间点肺组织病理变化。 结果 与A组比较，B组动脉血PO2和BALF中SP-A浓度降低显著（P 0.05），湿干比及单核巨噬细胞中IL-18表达增加（P 0.05），肺组织充血水肿明显。与C组相比，上述改变得以逆转，肺损伤程度明显减轻（P 0.05）。 结论 中介素1-53可通过抑制SP-A的减少和IL-18的生成在急性肺损伤早期起到一定的保护作用。 　　[关键词] 急性肺损伤；中介素1-53；SP-A；IL-18 　　[中图分类号] R563 [文献标识码] A [文章编号] 1673-9701（2012）03-0001-03 　　 　　Effects of intermedin 1-53 on SP-A and IL-18 in LPS-induced acute lung injury septic rats 　　LIU Ling1 LI Jianqiang2 SHI Jing1 ZHAO Hui2 　　1.Shanxi Medical University, Taiyuan 030001, China; 2.Department of Respiration, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China 　　[Abstract] Objective To explore the influence of intermedin1-53 on the leve of SP-A in BALF and IL-18 expressions of alveolar macrophages in lipopolysaccharide of LPS-induced acute lung injury (ALI) in rats. Methods All of 36 male Wistar rats were randomly divided into 3 groups: normal control (A), LPS-treated group (B), intermediary 1-53 intervention group (C). Blood samp leswere taken for blood gas analysis at 2, 4 h.The animals were killed after 4 h, the ratio of wet to dry lung weight was measured. The concentration of SP-A in BALF with ELISA method. The exression of IL-18 was examined by immunohistochemical method and the changes of lung tissue were pathol ogically observed. Results Compared with group A, PO2 and SP-A had a decreased significantly in 2 and 4 hours in group B (P 0.05）. The ratio of wet to dry lung weight and the expressi of IL-18 alveolar macrophages in lipopolysaccharide had a increased significantly in group B (P 0.05), The degree of lung injury was hardly. These changes were inverted in group C, and the degree of lung injury was markedly at ten