中药清毒栓对宫颈癌SiHa细胞产生免疫相关细胞因子影响.docVIP

中药清毒栓对宫颈癌SiHa细胞产生免疫相关细胞因子的影响 　　[摘要] 目的 探讨中药清毒栓含药血清对宫颈癌SiHa细胞产生免疫相关细胞因子的影响。方法 选取SPF级SD大鼠雌性30只[体重（220±10）g]，分成两组，分别用纯净水和中药灌胃处理后抽血提取含药血清，设立空白对照组（0%含药大鼠血清）、清毒栓低、中、高剂量组（浓度分别为1%、4%、8%含药大鼠血清），采用q-PCR法检测细胞IL-2、IL-8、TNF-α、IFN-γ的基因表达情况，采用Western Blot法检测SiHa细胞内IL-8、TNF-α的蛋白表达情况。结果 不同剂量的清毒栓含药血清在12 h、24 h、48 h对宫颈癌SiHa细胞免疫相关细胞因子的基因表达均有一定促进表达作用，其中以IL-8、TNF-α在8%浓度含药血清给药后12～24 h内呈现显著上升的趋势（P0.05）；细胞内IL-8、TNF-α蛋白表达量在高剂量组给药后48 h呈现显著上升的趋势。结论 高剂量清毒栓含药血清可显著诱导免疫相关细胞因子IL-8、TNF-α基因的表达，并能够使细胞内IL-8、TNF-α蛋白的表达量增加，其作用机制可能是通过促进相关基因的表达量来增加相应细胞因子蛋白的表达。 　　[关键词] 免疫相关细胞因子；SiHa细胞；中药清毒栓；含药血清 　　[中图分类号] R285 [文献标识码] A [文章编号] 1673-9701（2017）30-0033-04 　　[Abstract] Objective To investigate the effect of traditional Chinese medicine Qingdu suppository-containing serum on immune-related cytokines in SiHa cells of cervical cancer. Methods SPF grade SD rats（body weight of 220±10）g were selected， with 30 female rats， which were divided into two groups. The drug-containing serum was collected by blood drawing after the treatment of pure water and Chinese medicine gavage respectively. The blank control group （0% drug-containing rat serum）， low， medium and high dose group of Qingdu suppository （concentrations of 1%， 4%， 8% of drug-containing rat serum） were set. The gene expression of IL-2， IL-8， TNF-α and IFN-γ was detected by q-PCR method， and the protein expression of IL-8 and TNF-α within SiHa cells was detected by Western Blot method. Results Different doses of Qingdu suppository-containing serum all showed a certain effect of expression promotion on the gene expression of immune-related cytokines in SiHa cells of cervical cancer at 12 h， 24 h， 48 h. The level of IL-8 and TNF-α was in a significantly increasing trend within 12 to 24 hours after the administration of 8% drug-containing serum（P0.05）； the protein expression volume of IL-8 and TNF-α in the cells was in a significantly increasing trend within 48 hours after the administration in the high-dose group. Conclusion The Qingdu suppository-containing