唾液腺腺样囊性癌中Runx3表达及临床意义.docVIP

唾液腺腺样囊性癌中Runx3表达及临床意义 　　[摘要] 目的 探讨唾液腺腺样囊性癌（SACC）中Runx3的表达及与临床病理因素的相关性。 方法 采用荧光定量RT-PCR检测Runx3在6例SACC组织及正常唾液腺组织中的表达。免疫组化检测58例SACC和正常唾液腺组织中Runx3蛋白的表达。采用SPSS17.0软件包分析Runx3的表达程度与临床病理因素之间的相关性。 结果 qRT-PCR的测量显示唾液腺腺样囊性癌中Runx3 mRNA表达明显下调。免疫组化结果显示，Runx3在SACC中的表达与病理类型（实体型）、T临床分期及淋巴结转移显著相关，而与肿瘤远处转移存在弱相关。 结论 Runx3表达下调与SACC发生发展相关，可能是临床诊断和治疗SACC的重要生物标志物。 　　[关键词] Runx3；唾液腺；腺样囊性癌；RT-PCR 　　[中图分类号] R739.8 [文献标识码] A [文章编号] 1673-9701（2015）25-0001-03 　　Expression and clinical significance of Runx3 in salivary adenoid cystic carcinoma 　　XU Zhengyuan1 HE Jianfeng2 　　1.Department of Stomatology， Changxing Hospital of Traditional Chinese Medicine in Zhejiang Province， Changxing 313100， China； 2.Department of Stomatology， the First Affiliated Hospital of Medical College in Zhejiang University， Hangzhou 310000， China 　　[Abstract] Objective To investigate the correlation between the expression of Runx3 and the clinical pathologic factors in salivary adenoid cystic carcinoma （SACC）. Methods The fluorescent quantitation RT-PCR was used to detect the expression of Runx3 in 6 cases of SACC tissues and normal salivary gland tissues. The immunohistochemistry was used to detect the expression of Runx3 protein in 58 cases of SACC and normal salivary gland tissues. The SPSS 17.0 software package was used to analyze the correlation between the expression degree of Runx3 and the clinical pathological factors. Results The qRT-PCR measurement showed that the expression of Runx3 mRNA reduced significantly in SACC. The immunohistochemical results showed that the expression of Runx3 in SACC was significantly correlated to the pathological pattern（entity type）， T clinical stage and lymph node metastasis， but was slightly correlated to the distant metastasis of tumors. Conclusion Runx3 expression downregulation is correlated to the occurrence and development of SACC， which can be an important biomarker for the clinical diagnosis and treatment of SACC. 　　[Key words] Runx3； Salivary gland； Adenoid cystic