响应面法优化酶辅助提取佛手瓜总黄酮工艺研究.docVIP

响应面法优化酶辅助提取佛手瓜总黄酮工艺研究 　　摘 要：为提高佛手瓜总黄酮提取率，采用酶辅助法对佛手瓜总黄酮的提取效果进行了研究。以总黄酮提取率为指标，研究了酶种类、酶用量、酶解温度、pH和酶解时间对总黄酮提取率的影响，在此基础上，利用响应面法对酶辅助提取佛手瓜总黄酮工艺条件进行了优化，建立了二次多项式模型，确定了最佳工艺条件。结果表明，在试验的4种酶中，以纤维素酶为水解酶的提取效果最好。纤维素酶辅助提取佛手瓜总黄酮的最佳工艺条件为：酶用量1.0%，酶解温度47℃，pH5.2，酶解时间46min，该条件下总黄酮提取率为3.802%，与预测值相比，相对误差为0.653%，表明回归模型所得数据与实验结果符合良好，验证了数学模型的有效性。该工艺与热浸提法相比，总黄酮的提取率提高了25.9%，为佛手瓜总黄酮的深度开发与利用提供了科学依据。 　　关键词：佛手瓜；总黄酮；酶辅助；响应面；优化 　　中图分类号 TQ28；S38；TS255；Q814 文献标识码 A 文章编号 1007-7731（2017）14-0020-06 　　Abstract：In this work the enzymes-assisted technology was used for extraction total flavonoids from Sechium edule（Jacq.） Swartz. The effects of enzymes types，enzyme amount，enzymolysis temperature，pH and enzymolysis time on the yield of total flavonoids were studied. The response surface method had been used to further optimize the extraction process by an establishment of quadratic polynomial regression equation for optimal extraction condition. The results showed that the cellulase was the best enzyme with the highest total flavonoids extraction yield，Furthermore，the optimal extraction conditions of total flavonoids were as follows： enzyme amount 1.0%，enzymolysis temperature 47 ℃，pH 5.2，enzymolysis time 46 min. The yield of total flavonoids was 3.802% and the relative error between the theoretic values （3.827%） and testing values （3.802%） was 0.653%，which indicated the obtained data were in good agreement with the experimental results and the feasible model fitted well with the experimental data. Meanwhile the extraction yield increased by 25.9% compared with the traditional extraction method. Results could provide scientific foundation for the exploitation and utilization of the total flavonoids in Sechium edule（Jacq.） Swartz. 　　Key words：Sechium edule（Jacq.） Swartz；Total flavonoids；Enzyme-assisted；Response surface methodology；Optimization 　　佛手瓜（Sechium edule（Jacq.）Swartz）又名拳?^瓜、万年瓜，为葫芦科佛手瓜属多年草本植物，佛手瓜味美可口、清脆多汁，是一种富含营养的珍稀蔬菜[1]。佛手瓜原产于西印度群岛、墨西哥一带，于20世纪初引入我国，由于其适应性强、高产质优、效益高，在我国的福建、广东、广西、云南等地区已广泛栽种[2]。佛手瓜