姜黄素对大鼠肝星状细胞作用研究.docVIP

姜黄素对大鼠肝星状细胞作用研究 　　[摘要]目的 探?姜黄素对大鼠肝纤维化防治作用的机制，为临床治疗提供参考。方法 建立CCl4致大鼠实验性肝纤维化的模型，同时每只大鼠按每100克体重分别给予20、10、5 mg姜黄素进行灌胃处理，相应设定为模型给药组、模型组以及对照组，每组各10只大鼠。比色法检测血清组织内活性氧类物质（ROS）水平；丙二醛（MDA）测定试剂盒（TBA法）检测肝组织MDA含量；凝胶迁移实验（EMSA）检测肝组织NF-κB活性；免疫组织化学法检测肝组织中NF-κB表达、转化生长因子-β超家族（TGF-β1）、血小板衍生生长因子-BB（PDGF-BB）、重组人结缔组织生长因子（CTGF）水平；RT-PCR法检测肝组织基质金属蛋白酶抑制剂-1（TIMP-1）的mRNA水平。并对各组检测结果进行统计分析。结果 模型组大鼠的ROS、内毒素、MDA、NF-κB、TGF-β1、PDGF-BB、CTGF、TIMP-1mRNA水平均明显高于对照组，差异有统计学意义（P0.05）；模型给药组大鼠的上述因子水平均低于模型组，差异有统计学意义（P0.05）。结论 姜黄素对血清中ROS及内毒素水平，肝组织中MDA、NF-κB、TGF-β1、PDGF-BB、CTGF、TIMP-1mRNA表达均有不同程度的抑制作用，其通过多种机制参与到预防肝纤维化的过程中。 　　[关键词]姜黄素；大鼠；肝纤维化；肝星状细胞；增殖；凋亡 　　[中图分类号] R575.204 [文献标识码] A [文章编号] 1674-4721（2017）11（c）-0004-03 　　Study of Curcumin on hepatic stellate cells in rats 　　WU Xiong-jian1 ZHENG Hong2 ZHU Hai-yan1 MAO Zhong-yi1 XIE Jun1 　　1.Department of Gastroenterology，the First Affiliated Hospital of Gannan Medical College，Ganzhou 341000，China；2.Gannan Medical College，Jiangxi Province，Ganzhou 341000，China 　　[Abstract]Objective To explore the mechanism of Curcumin on the prevention and treatment of liver fibrosis in rats，so as to provide reference for clinical treatment.Methods The rat model of experimental liver fibrosis induced by CCl4 was established.At the same time，20 mg，10 mg and 5 mg Curcumin were in lavage in every 100 g body weight，the corresponding models were set as delivery model group，model group and control group respectively，10 rats in each group.The level of reactive oxygen species （ROS） in serum was detected by colorimetric method.Malondialdehyde （MDA） content in liver tissue was detected by MDA assay kit （TBA method）.By electrophoretic mobility shift assay （EMSA），the activity of NF-κB in liver tissue was detected.The levels of NF-κB expression，TGF-β1，platelet-derived growth factor-BB （PDGF-BB） and recombinant human connective tissue growth factor （CTGF） in liver tissue were detected by immunohistochemistry.The mRNA level of tissue inhibitor of metalloproteinase-1 in liver tissue was detec