Tim3参与巨噬细胞炎症反应的机制及对巨噬细胞抗原呈递作用的分析-免疫学专业论文.docxVIP

between Tim3 and TLR4 associated with AKT、NF-kB signaling pathways. We deduced that greater phophorylation of AKT can significantly inhibit the activation of NF-kB, which decreased TNF-α production in response to LPS stimulation. (3) In sepsis mouse models, our data shown that the up-regulated expression of Tim3 in early stage of sepsis which mediated pro-inflammatory. However, the expression of Tim3 is deregulated in late sepsis. (4) Oue in vivo and vitro data showed that Tim3 also regulates the expression of the co-stimulatory molecules CD80 and CD86 on macrophage, and thus promotes Th1, Th17 polarization by improving IFN-γ and IL-17 production. Conclusions: (1) ELISA data showed that activation of Tim3 pathway enhanced TNF-α and IL-6 secretion from macrophage, which suggest that Tim3 is especially important for regulating innate immune response. (2) Our data suggested that there is an antagonistic effect between Tim3 and TLR4 pathway. (3) The results showed that Tim-3, by increasing CD86 and decreasing CD80, promote Th1、Th17 polarization. (4) Our dates provide new information concerning the role of Tim3 in innate and adaptive immunity, especially provide valuable knowledge about the regulatory roles of Tim3 in macrophage mediated immune response. KEY WORDS: Tim3, macrophage, innate and adaptive immunity IV 缩略词表 APC antigen presenting cells 抗原提呈细胞 CD cluster of differentiation 分化群 CLP Cecal ligation puncture 盲肠结扎穿孔 DMEM dulbecco minimum essential medium 极限必需培养基 DMSO dimethyl sulfoxide 二甲基亚砜 ECL enhanced chemi-luminescence 增敏化学发光显色 ELISA enzyme linked immunosorbent assay 酶联免疫吸附测定 FACS Fluorescence activated cell sorting 流式细胞术 FCS Fetal cattle serum 胎牛血清 GFP Green fluorescent protein 绿色荧光蛋白 Gal-9 Galectin-9 半乳凝素-9 HRP horseradish peroxidase 辣根过氧化物酶 IFN-γ Interfron-γ 干扰素-r IL Interleukin 白细胞介素 LPS Lipopolysaccharide 细菌脂多糖 NC Negative control 阴性对照 OD optical density 光密度值 PCR polymerase chain reaction 聚合酶链反应 PAGE polyacrylamide gel electrophoresis 聚丙烯酰胺凝胶电泳