TLR2在皮肤癣菌感染中对角质形成细胞分泌γ-IFN和IL-8影响-皮肤性病学专业论文.docxVIP

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 5 5 Section Ⅱ Objective Observe the change of γ-IFN and IL-8 secreted by keratinocytes after Trichophyton rubrum infection, and the effect of TLR2 on γ-IFN and IL-8 secretion. To explore the role TLR2 in anti-dermatophyte infection. Methods Keratinocytes treated before and after anti-TLR2 monoantibody were stimulated by Trichophyton rubrum .The levels ofγ-IFN and IL-8 in supernatant were determined with ELISA at different time points. Results The levels of γ-IFN and IL-8 secreted by kerainocytes were significantly increased after induced with Trichophyton rubrum(P﹤0.05).The level of γ-IFN reached (85.36±4.54)pg/ml at the point of 4h,and （445.58±13.99）pg/ml at 16h; After treated with anti-TLR2 monoantibody, the levels of IL-8 were reduced at the points of 2h,4h,8h and 16h(P﹤0.05); and the levels of γ-IFN were reduced at the points of 2h, 4h and 8h(P﹤0.05), but the difference at the point of 16h was not statistically significant(P﹥0.05). Conclusion After stimulated keratinocytes, Trichophyton rubrum can promote its secretion of γ-IFN and IL-8; TLR2 play an important role onγ-IFN and IL-8 secretion of keratinocytes. Key words keratinocyte; Trichophyton rubrum; TLR2; γ-IFN；IL-8 1 1 英文缩略词表 英文缩写 TLR 英文全称 Toll-like receptor 中文名称 Toll 样受体 γ-IFN γ-interferon γ-干扰素 IL Interleukin 白介素 FITC Fluoresceine isothiocyanate 异硫氰酸 PAMPs Pathogen associated molecular patterns 病原相关分子模式 PRRs Pattern recognition receptors 模式识别受体 KcMR Keratinocytemannose-binding receptor 角质形成细胞甘露糖结合 受体 NF-kB Nuclear factor-kappa B 核转录因子-kB iNO Inducible nitric oxide synthese enzyme 诱生型一氧化氮合酶 LPS Lipopolysaccharide 脂多糖 TNF-α Tumor necrosis factor 肿瘤坏死因子 DC Dentric cell 树突状细胞 无血清角质形成细胞培养 KC-SFM Keratinocyte Serun-Free Medium 基 BPE Bovine Pituitary Extract 牛脑垂体提取物 rEGF Recombinant Epidermal Growth Factor 重组表皮生长因子 CR Cysteine-rich domain 富含半胱氨酸区域 FNII Fibronectin type II domain II 型纤连蛋白重复区 CTLD C-type lectin-likedomain C 型凝集素样糖类识别域 TIR Toll IL 1 receptor domain Toll IL 1 受体