Th17细胞在动脉粥样硬化发病中的作用及全反式维甲酸对其影响-生物化学与分子生物学专业论文.docxVIP

PAGE PAGE 2 验中高脂组及免疫高脂组大鼠血清总胆固醇值较正常组、免疫组增加有显著性差 异( P 0.05) ，而 ATRA 组与各组间差异无统计学意义。HE 染色显示免疫高脂组 动脉内膜增生明显，内皮下有大量泡沫细胞沉积，免疫组及 ATRA 组动脉病变程 度比免疫高脂组减轻，正常组及高脂组动脉内膜连续完整。免疫组及免疫高脂组 大鼠 Th17 细胞表达率与正常组及高脂组相比有明显增高( P0.05) ，ATRA 治疗组 Th17 细胞表达率与免疫高脂组比较明显降低( P0.05) 。结论 ACS 患者外周 Th17 细胞表达率、IL-17、ox-LDL 及 RORγt 表达水平增加，Th17 细胞可能参与动脉粥 样斑块不稳定和急性冠状动脉综合征的发病。Ox-LDL 可增加外周 Th17 细胞表达 率而 ATRA 可抑制这一效应。ATRA 可抑制 Th17 细胞的诱导分化而 ox-LDL 对 Th17 细胞的诱导分化无明显影响。早期 AS 大鼠模型建立成功，早期 AS 大鼠外 周 Th17 细胞表达率明显升高，ATRA 可降低其 Th17 细胞表达率并减轻 AS 病变 程度。 关键词 动脉粥样硬化 Th17 细胞 氧化低密度脂蛋白 全反式维甲酸 The roles of Th17 cells in atherosclerosis and the effects of all-trans-retinoic- acid on this process Student Wang Yi Advisor Wang Yuan Abstract Objective To investigate the roles of T help 17 cells in atherosclerosis（AS）and to study the effects of ATRA on this process. Methods In the first part of experiment ,the participants in the study were patients with chest pain syndrome (CPS), stable angina (SA), unstable angina（UA）, acute myocardial infarction(AMI). The frequencies of Th17 in peripheral blood, the serum levels of IL-17 and ox-LDL, and the levels of RORγt were measured by FCM, ELISA, and RT-PCR respectively. The frequencies of Th17 in peripheral blood of patients with CPS were measured by FCM after being stimulated with ox-LDL at different concentrations for 24 hours and 48 hours.FCM were used to measure the effects of ATRA on expression of Th17 cells treated with ox-LDL in patients of 4 groups. Naive T cells were separated from CPS and cultured in vitro. Cytokines IL-1β , IL-6 ,IL-23were used to induce naive T cells to develop into Th17 cells, the effects of ATRA and ox-LDL on development of Th17 cells were study.In the second part of experiment,immune injury and high-fat feeding being used to establish a rat AS model. 30 Sprague Dawley rats were randomly divided into 5 groups: normal group, high-cholesterol group, immune-injured group, high-cholesterol and immune-injured group, ATRA group.Serum cholesterol were analyzed. H