活动性肺结核患者基质金属蛋白酶9和血管内皮细胞生长因子在血清中表达及其临床意义.docVIP

活动性肺结核患者基质金属蛋白酶9和血管内皮细胞生长因子在血清中表达及其临床意义 　　【摘要】目的: 探讨基质金属蛋白酶-9(matrix metalloproteinase-9, MMP-9)及血管内皮细胞生长因子(vascular endothelial growth factor, VEGF)在活动性肺结核患者中的表达及其临床意义。方法: 采用ELISA方法检测42例活动性肺结核患者及42例健康人血清中MMP-9、VEGF表达水平。结果:活动性肺结核患者血清MMP-9、VEGF水平显著高于健康对照者(P0.001);痰结核杆菌涂片阳性患者血清中MMP-9、VEGF水平显著高于涂片阳性患者(P0.001);肺内广泛病变患者血清中MMP-9、VEGF水平显著高于肺内局限病变患者(P0.001)。结论: MMP-9、VEGF的表达水平可能与活动性肿结核的发生发展有关,并且与肺结核临床特点密切相关。 　　【关键词】肺结核;基质金属蛋白酶-9;血管内皮细胞生长因子 　　 　　Investigation of the serum MMP-9 and VEGF and its role in active pulmonary tuberculosis patients 　　HE Qin?Wei 　　【Abstract】Objective To study the relationship between the serum expression of MMP-9 and VEGF with the clinical characteristics in active pulmonary tuberculosis patients. Methods Expression of MMP-9 and VEGF were detected by ELISA method in 42 patients with active pulmonary tuberculosis and with a control of 42 health cases. Results The serum levels of MMP-9 and VEGF in active pulmonary tuberculosis patients were significantly higher than that of healthy group(P0.001). The serum levels of MMP-9 and VEGF in patients with advanced disease, sputum positive patients and pulmonary cavity patients were significantly higher than that in those with limited disease, sputum negative andwithout cavity respectively in active pulmonary tuberculosis patients (P0.001). Conclusion: Our data suggest the serum levels of MMP-9 and VEGF play an important role in active pulmonary tuberculosis patients and have close relation with clinical characteristics in active pulmonary tuberculosis patients. 　　【Key words】Pulmonary tuberculosis;Matrix metalloproteinase-9;Vascular endothelial growth factor 　　【中图分类号】R521 【文献标识码】A 【文章编号】1006-1959(2009)08-0045-02 　　 　　结核是威胁人类健康的重要疾病之一。目前全世界每年结核发病率以大于1%的速度增长,即全世界每年有800万的新增结核病例,而每年有200万人直接死于结核病[1]。结核发病率的增加除与现在流行的结核菌株抗菌性增加有关外,对结核感染机制可能由T淋巴细胞介导的细胞免疫反应,多种炎症性细胞因子和血管生成因子参与了该过程[1]。本研究即利用双抗体夹心ELISA法检测活动性肺结核患者血清中MMP-9及VEGF的表达,探讨其在肺结核发生发展中的作用及与临床特点的关系。 　　 　　1 病例与方法 　　 　　1.1病例: 　　活动性肺结核患者42名。近2个月内无其它感染性疾病,其中