沼渣肥对蓝莓土壤微生物多样性影响.docVIP

沼渣肥对蓝莓土壤微生物多样性影响 　　摘要：采用磷脂脂肪酸（PLFA）生物标记法分析研究了沼渣肥对蓝莓（Vaccinium spp.）根际土壤微生物多样性的影响。结果表明，用沼渣肥和草炭处理蓝莓土壤PLFA标记含量存在差异，其中用沼肥处理的蓝莓土壤的PLFA总量最大；与未做任何处理和用草炭处理的相比，用沼渣肥处理的蓝莓土壤中多两个细菌类群，分别是i15∶0（好氧细菌）和a16∶0（革兰氏阳性细菌）；通过对不同处理的蓝莓土壤养分含量进行分析发现，添加了沼渣肥的蓝莓土壤有机质、速效钾、有效磷、全氮的含量最高；通过相关性分析可知，蓝莓土壤中微生物生长需要有效磷、有机质、速效钾，特别是腐生真菌、AM真菌、18∶1ω7（假单胞杆菌）等对蓝莓生长有益的微生物更需要有效磷。 　　关键词：蓝莓（Vaccinium spp.）；根际土壤；沼渣肥；微生物群落；磷脂脂肪酸标记（PLFA） 　　中图分类号：S141.4；S666 文献?耸堵耄?A 文章编号：0439-8114（2017）19-3630-06 　　DOI：10.14088/j.cnki.issn0439-8114.2017.19.010 　　Abstract： The microbial diversity was analyzed in the rhizophere of blueberries（Vaccinium spp.） which treated with biogas residue fertilizer by using phospholipid fatty acid biomarkers（PLFA）. The results showed that there were obvious differences in the contents of PLFA in the rhizophere soils treated with biogas residue fertilizer and grass carbon，and the total amount of PLFA in blueberries soil treated with biogas residue fertilizer was the highest. Compared with the soil without any treatment and the soil with grass carbon treatment，the rhizosphere of blueberries soil treated with biogas residue fertilizer has another two bacteria groups：i15∶0（aerobic bacteria） and a16∶0（G+）. According to the nutrient content analysis of blueberries soils by different treatments，the study found that the blueberries soil added by biogas residue fertilizer had the highest contents of organic matter，available potassium， available phosphorus and total nitrogen. The results of correlation analysis indicated that microbial growth in blueberries soil required effective phosphorus， organic matter and available potassium，especially saprophytic fungi，AM fungi，18∶1ω7（pseudomonas） and other beneficial spices required more available potassium. 　　Key words： blueberries（Vaccinium spp.）； rhizosphere soil； biogas residue fertilizer； microbial community； phospholipid fatty acid marker （PLFA） 　　应用厌氧发酵生产沼气的研究已近100年，在中国随着沼气建设事业的蓬勃发展，自20世纪80年代以来，沼肥的再利用已成为人们普遍关注的问题[1]。沼肥是将畜禽废弃物、人畜粪便、有机生活垃圾和作物秸秆等按一定比例混合接种特